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In vivo imaging and detection of nitroreductase in zebrafish by a new near-infrared fluorescence off-on probe

Li, Z.; He, X.; Wang, Z.; Yang, R.; Shi, W.; Ma, H.; Biosens. Bioelectron. 63, 112-116 (2015)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
fluorescence imaging of nitroreductase in zebrafish in vivo using a newnear-infrared fluorescence off-on probe. The probe is prepared by connecting 4-nitrobenzene as a quenching and recognizing moiety to a stable hemicyanine skeleton. The fluorescence off-on response of the probe is based on the enzyme-catalyzed reduction of the 4-nitrobenzene moiety, followed by the 1,6-rearrangement-elimination and the fluorophore release. The probe exhibits good analytical performance such as near-infrared fluorescence emission over 700 nm as well as high selectivity and sensitivity, with a detection limit of 14 ng/ml. The probe has been applied to visualize the distribution of nitroreductase in living zebrafish in vivo
Danio rerio
Cloned(Commentary)
Cloned (Commentary)
Organism
-
Danio rerio
Organism
Organism
UniProt
Commentary
Textmining
Danio rerio
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
yolk sac
main localization
Danio rerio
-
Application (protein specific)
Application
Commentary
Organism
analysis
fluorescence imaging of nitroreductase in zebrafish in vivo using a newnear-infrared fluorescence off-on probe. The probe is prepared by connecting 4-nitrobenzene as a quenching and recognizing moiety to a stable hemicyanine skeleton. The fluorescence off-on response of the probe is based on the enzyme-catalyzed reduction of the 4-nitrobenzene moiety, followed by the 1,6-rearrangement-elimination and the fluorophore release. The probe exhibits good analytical performance such as near-infrared fluorescence emission over 700 nm as well as high selectivity and sensitivity, with a detection limit of 14 ng/ml. The probe has been applied to visualize the distribution of nitroreductase in living zebrafish in vivo
Danio rerio
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Danio rerio
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
yolk sac
main localization
Danio rerio
-
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740989
Yang
Heterologous overexpression an ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Mol. Biotechnol.
58
428-440
2016
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9
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740192
Li
In vivo imaging and detection ...
Danio rerio
Biosens. Bioelectron.
63
112-116
2015
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Yuan
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Pseudomonas sp., Pseudomonas sp. N1
Chin. J. Appl. Environ. Biol.
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1031-1034
2013
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Isayev
In silico structure-function a ...
Enterobacter cloacae
Proteins
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2728-2741
2012
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741093
Yanto
Nitroreductase from Salmonella ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Org. Biomol. Chem.
8
1826-1832
2010
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6
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3
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1
1
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677659
Wu
Novel partial reductive pathwa ...
Comamonas sp., Comamonas sp. Ctestosteroni
Appl. Environ. Microbiol.
72
1759-1765
2006
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1
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677735
Xiao
Characterization of genes invo ...
Pseudomonas putida, Pseudomonas putida ZWL73
Appl. Microbiol. Biotechnol.
73
166-171
2006
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1
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7
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14
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14
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740133
Berne
Application of a microfluidic ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
Biomacromolecules
7
2631-2636
2006
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1
1
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6
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740929
Ho
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Characterization of nitroreduc ...
Pseudomonas sp. HK-6
Korean J. Microbiol. Biotechnol.
32
230-237
2004
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5
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5
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740596
Somerville
Purification and characterizat ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
J. Bacteriol.
177
3837-3842
1995
1
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11
3
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4
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4
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1
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8
1
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3
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11
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8
1
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739974
Rieble
Aromatic nitroreductase from t ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
205
298-304
1994
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