BRENDA - Reference to 1.7.1.16; Id = 740133

analysis

immobilization of enzyme on polyethyleneimine-mediated silica formation, enzyme activity is significantly more stable than NbzA in solution. A microfluidic microreactor is suitable for continuous operation using nitrobenzene, CB1954, and the proantibiotic nitrofurazone. The flow-through system provides a rapid and reproducible screening method for determining the NbzA-catalyzed activation of prodrugs and proantibiotics

Pseudomonas pseudoalcaligenes

expression in Escherichia coli

Pseudomonas pseudoalcaligenes

0.002

-

nitrobenzene

imobilized enzyme, 25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

0.0023

-

nitrobenzene

25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

0.0117

-

5-(aziridin-1-yl)-2,4-dinitrobenzamide

25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

0.0337

-

5-(aziridin-1-yl)-2,4-dinitrobenzamide

imobilized enzyme, 25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

1.763

-

nitrofurazone

25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

5.123

-

nitrofurazone

imobilized enzyme, 25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

Q6DLR9

-

Pseudomonas pseudoalcaligenes JS45

Q6DLR9

-

Pseudomonas pseudoalcaligenes

5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes

? + 2 NADP+ + H2O

enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide

-

?

5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes JS45

? + 2 NADP+ + H2O

enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide

-

?

nitrobenzene + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes

N-hydroxyaniline + 2 NADP+ + H2O

-

?

nitrobenzene + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes JS45

N-hydroxyaniline + 2 NADP+ + H2O

-

?

nitrofurazone + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes

? + 2 NADP+ + H2O

enzyme activates the proantibiotic nitrofurazone

-

?

nitrofurazone + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes JS45

? + 2 NADP+ + H2O

enzyme activates the proantibiotic nitrofurazone

-

?

NbzA

-

Pseudomonas pseudoalcaligenes

analysis

immobilization of enzyme on polyethyleneimine-mediated silica formation, enzyme activity is significantly more stable than NbzA in solution. A microfluidic microreactor is suitable for continuous operation using nitrobenzene, CB1954, and the proantibiotic nitrofurazone. The flow-through system provides a rapid and reproducible screening method for determining the NbzA-catalyzed activation of prodrugs and proantibiotics

Pseudomonas pseudoalcaligenes

expression in Escherichia coli

Pseudomonas pseudoalcaligenes

0.002

-

nitrobenzene

imobilized enzyme, 25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

0.0023

-

nitrobenzene

25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

0.0117

-

5-(aziridin-1-yl)-2,4-dinitrobenzamide

25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

0.0337

-

5-(aziridin-1-yl)-2,4-dinitrobenzamide

imobilized enzyme, 25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

1.763

-

nitrofurazone

25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

5.123

-

nitrofurazone

imobilized enzyme, 25°C, pH not specified in the publication

Pseudomonas pseudoalcaligenes

-

Pseudomonas pseudoalcaligenes

5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes

? + 2 NADP+ + H2O

enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide

-

?

5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes JS45

? + 2 NADP+ + H2O

enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide

-

?

nitrobenzene + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes

N-hydroxyaniline + 2 NADP+ + H2O

-

?

nitrobenzene + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes JS45

N-hydroxyaniline + 2 NADP+ + H2O

-

?

nitrofurazone + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes

? + 2 NADP+ + H2O

enzyme activates the proantibiotic nitrofurazone

-

?

nitrofurazone + 2 NADPH + 2 H+

-

740133

Pseudomonas pseudoalcaligenes JS45

? + 2 NADP+ + H2O

enzyme activates the proantibiotic nitrofurazone

-

?

740989

Yang

Heterologous overexpression an ...

Gluconobacter oxydans, Gluconobacter oxydans 621H

Mol. Biotechnol.

58

428-440

2016

-

7

9

-

2

-

1

-

13

-

9

-

1

-

1

-

7

-

9

-

1

-

13

-

9

-

9

740192

Li

In vivo imaging and detection ...

Danio rerio

Biosens. Bioelectron.

63

112-116

2015

-

1

-

1

-

1

-

1

-

1

-

740325

Yuan

-

Isolation and characterization ...

Pseudomonas sp., Pseudomonas sp. N1

Chin. J. Appl. Environ. Biol.

19

1031-1034

2013

-

1

-

2

-

2

-

1

-

2

-

741380

Isayev

In silico structure-function a ...

Enterobacter cloacae

Proteins

80

2728-2741

2012

-

1

-

1

-

1

-

1

-

1

-

741093

Yanto

Nitroreductase from Salmonella ...

Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2

Org. Biomol. Chem.

8

1826-1832

2010

-

2

-

2

-

6

-

1

-

3

-

1

-

2

-

6

-

3

-

1

-

677659

Wu

Novel partial reductive pathwa ...

Comamonas sp., Comamonas sp. Ctestosteroni

Appl. Environ. Microbiol.

72

1759-1765

2006

-

1

-

2

-

2

-

1

-

1

-

1

-

2

-

1

-

677735

Xiao

Characterization of genes invo ...

Pseudomonas putida, Pseudomonas putida ZWL73

Appl. Microbiol. Biotechnol.

73

166-171

2006

-

1

-

7

-

14

-

1

-

2

-

1

2

-

14

-

740133

Berne

Application of a microfluidic ...

Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45

Biomacromolecules

7

2631-2636

2006

-

1

-

6

-

4

-

1

-

6

-

1

-

1

-

6

-

1

-

6

-

740929

Ho

-

Characterization of nitroreduc ...

Pseudomonas sp. HK-6

Korean J. Microbiol. Biotechnol.

32

230-237

2004

-

5

-

1

-

1

-

1

-

1

-

2

1

-

1

-

1

-

5

-

1

-

1

-

1

-

2

1

-

1

-

740596

Somerville

Purification and characterizat ...

Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45

J. Bacteriol.

177

3837-3842

1995

1

-

11

3

-

2

4

-

4

-

1

-

1

-

8

1

-

3

-

1

-

3

-

11

-

3

-

2

4

-

1

-

1

-

8

1

-

739974

Rieble

Aromatic nitroreductase from t ...

Phanerochaete chrysosporium

Biochem. Biophys. Res. Commun.

205

298-304

1994

-

1

-

1

-

1

-

2

-

7

-

1

-

1

-

1

-

1

-

2

-

7

-

1

-

1

-

Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase

Data extracted from this reference: