BRENDA - Enzyme Database
show all sequences of 1.7.1.16

Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase

Berne, C.; Betancor, L.; Luckarift, H.R.; Spain, J.C.; Biomacromolecules 7, 2631-2636 (2006)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
immobilization of enzyme on polyethyleneimine-mediated silica formation, enzyme activity is significantly more stable than NbzA in solution. A microfluidic microreactor is suitable for continuous operation using nitrobenzene, CB1954, and the proantibiotic nitrofurazone. The flow-through system provides a rapid and reproducible screening method for determining the NbzA-catalyzed activation of prodrugs and proantibiotics
Pseudomonas pseudoalcaligenes
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Pseudomonas pseudoalcaligenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
nitrobenzene
imobilized enzyme, 25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
0.0023
-
nitrobenzene
25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
0.0117
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
0.0337
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
imobilized enzyme, 25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
1.763
-
nitrofurazone
25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
5.123
-
nitrofurazone
imobilized enzyme, 25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas pseudoalcaligenes
Q6DLR9
-
-
Pseudomonas pseudoalcaligenes JS45
Q6DLR9
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas pseudoalcaligenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes
? + 2 NADP+ + H2O
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes JS45
? + 2 NADP+ + H2O
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes JS45
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrofurazone + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes
? + 2 NADP+ + H2O
enzyme activates the proantibiotic nitrofurazone
-
-
?
nitrofurazone + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes JS45
? + 2 NADP+ + H2O
enzyme activates the proantibiotic nitrofurazone
-
-
?
Synonyms
Synonyms
Commentary
Organism
NbzA
-
Pseudomonas pseudoalcaligenes
Application (protein specific)
Application
Commentary
Organism
analysis
immobilization of enzyme on polyethyleneimine-mediated silica formation, enzyme activity is significantly more stable than NbzA in solution. A microfluidic microreactor is suitable for continuous operation using nitrobenzene, CB1954, and the proantibiotic nitrofurazone. The flow-through system provides a rapid and reproducible screening method for determining the NbzA-catalyzed activation of prodrugs and proantibiotics
Pseudomonas pseudoalcaligenes
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas pseudoalcaligenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
nitrobenzene
imobilized enzyme, 25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
0.0023
-
nitrobenzene
25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
0.0117
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
0.0337
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
imobilized enzyme, 25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
1.763
-
nitrofurazone
25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
5.123
-
nitrofurazone
imobilized enzyme, 25°C, pH not specified in the publication
Pseudomonas pseudoalcaligenes
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas pseudoalcaligenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes
? + 2 NADP+ + H2O
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes JS45
? + 2 NADP+ + H2O
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes JS45
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrofurazone + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes
? + 2 NADP+ + H2O
enzyme activates the proantibiotic nitrofurazone
-
-
?
nitrofurazone + 2 NADPH + 2 H+
-
740133
Pseudomonas pseudoalcaligenes JS45
? + 2 NADP+ + H2O
enzyme activates the proantibiotic nitrofurazone
-
-
?
Other publictions for EC 1.7.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740989
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-
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7
9
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1
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13
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9
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1
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1
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7
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9
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1
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13
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9
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9
9
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63
112-116
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1
1
-
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-
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1
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1
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1
1
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1
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740325
Yuan
-
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Pseudomonas sp., Pseudomonas sp. N1
Chin. J. Appl. Environ. Biol.
19
1031-1034
2013
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
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-
-
-
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2
-
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1
1
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-
2
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-
-
-
-
-
-
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741380
Isayev
In silico structure-function a ...
Enterobacter cloacae
Proteins
80
2728-2741
2012
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
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-
-
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1
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-
1
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-
1
1
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-
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-
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-
-
-
-
741093
Yanto
Nitroreductase from Salmonella ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Org. Biomol. Chem.
8
1826-1832
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
6
-
1
-
-
3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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-
-
-
-
-
-
-
2
-
6
-
-
-
3
-
1
1
-
-
-
-
-
-
-
-
677659
Wu
Novel partial reductive pathwa ...
Comamonas sp., Comamonas sp. Ctestosteroni
Appl. Environ. Microbiol.
72
1759-1765
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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-
-
2
-
1
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1
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1
1
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-
-
2
-
-
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-
-
-
-
-
1
1
-
-
-
677735
Xiao
Characterization of genes invo ...
Pseudomonas putida, Pseudomonas putida ZWL73
Appl. Microbiol. Biotechnol.
73
166-171
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
14
-
1
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-
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-
-
-
2
-
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-
1
2
-
-
-
-
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-
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14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740133
Berne
Application of a microfluidic ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
Biomacromolecules
7
2631-2636
2006
-
1
1
-
-
-
-
6
-
-
-
-
-
4
-
-
1
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
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-
1
1
-
-
-
-
-
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-
6
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740929
Ho
-
Characterization of nitroreduc ...
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Korean J. Microbiol. Biotechnol.
32
230-237
2004
-
-
-
-
-
-
5
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
2
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
740596
Somerville
Purification and characterizat ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
J. Bacteriol.
177
3837-3842
1995
1
-
-
-
-
-
11
3
-
-
2
4
-
4
-
-
1
-
-
-
1
-
8
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
3
-
-
-
-
11
-
3
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739974
Rieble
Aromatic nitroreductase from t ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
205
298-304
1994
-
-
-
-
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1
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1
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1
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-
2
-
7
-
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1
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-
1
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1
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1
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2
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7
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1
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1
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