Aromatic nitroreductase from the basidiomycete Phanerochaete chrysosporium
Rieble, S.; Joshi, D.K.; Gold, M.H.; Biochem. Biophys. Res. Commun. 205, 298-304 (1994) 
Data extracted from this reference:
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated
Phanerochaete chrysosporium
16020
-
Organism
Organism
UniProt
Commentary
Textmining
Phanerochaete chrysosporium
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
membrane-associated enzyme, can be solubilized using Triton X-100
Phanerochaete chrysosporium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1900
-
cosubstrate NADH, pH 7.0, 47°C
Phanerochaete chrysosporium
3600
-
cosubstrate NADPH, pH 7.0, 47°C
Phanerochaete chrysosporium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
1-amino-3-nitrobenzene + 2 NADP+ + H2O
about 90% 1-amino-3-nitrobenzene plus 10% 1-hydroxylamino-3-nitrobenzene
-
-
?
1-hydroxylamino-3-nitrobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
1-amino-3-nitrobenzene + 2 NADP+ + H2O
-
-
-
?
1-nitroso-3-nitrobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
? + 2 NADP+ + H2O
only the amino product can be observed
-
-
?
2,4,6-trinitrotoluene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
? + 2 NADP+ + H2O
products are hydroxylamino- and amino-dinitrotoluene isomersd
-
-
?
2,4-dichloro-1-nitrobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
2,4-dichloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
? + 2 NADP+ + H2O
-
-
-
?
2,4-dinitrotoluene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
? + 2 NADP+ + H2O
most abundant products are amino-nitrotoluene isomers, plus some hydroxylamino and diaminotoluene isomers
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Phanerochaete chrysosporium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Phanerochaete chrysosporium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated
Phanerochaete chrysosporium
16020
-
Purification (Commentary) (protein specific)
Commentary
Organism
membrane-associated enzyme, can be solubilized using Triton X-100
Phanerochaete chrysosporium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1900
-
cosubstrate NADH, pH 7.0, 47°C
Phanerochaete chrysosporium
3600
-
cosubstrate NADPH, pH 7.0, 47°C
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
1-amino-3-nitrobenzene + 2 NADP+ + H2O
about 90% 1-amino-3-nitrobenzene plus 10% 1-hydroxylamino-3-nitrobenzene
-
-
?
1-hydroxylamino-3-nitrobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
1-amino-3-nitrobenzene + 2 NADP+ + H2O
-
-
-
?
1-nitroso-3-nitrobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
? + 2 NADP+ + H2O
only the amino product can be observed
-
-
?
2,4,6-trinitrotoluene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
? + 2 NADP+ + H2O
products are hydroxylamino- and amino-dinitrotoluene isomersd
-
-
?
2,4-dichloro-1-nitrobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
2,4-dichloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
? + 2 NADP+ + H2O
-
-
-
?
2,4-dinitrotoluene + 2 NADPH + 2 H+
-
739974
Phanerochaete chrysosporium
? + 2 NADP+ + H2O
most abundant products are amino-nitrotoluene isomers, plus some hydroxylamino and diaminotoluene isomers
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Phanerochaete chrysosporium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Phanerochaete chrysosporium
Other publictions for EC 1.7.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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13
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9
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9
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1
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2
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741380
Isayev
In silico structure-function a ...
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Proteins
80
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2012
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1
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1
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Org. Biomol. Chem.
8
1826-1832
2010
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6
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1
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3
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1
1
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2
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6
-
-
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3
-
1
1
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677659
Wu
Novel partial reductive pathwa ...
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Appl. Environ. Microbiol.
72
1759-1765
2006
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1
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2
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1
1
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677735
Xiao
Characterization of genes invo ...
Pseudomonas putida, Pseudomonas putida ZWL73
Appl. Microbiol. Biotechnol.
73
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2006
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14
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740133
Berne
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Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
Biomacromolecules
7
2631-2636
2006
-
1
1
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6
-
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4
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1
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6
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1
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1
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6
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740929
Ho
-
Characterization of nitroreduc ...
Pseudomonas sp. HK-6
Korean J. Microbiol. Biotechnol.
32
230-237
2004
-
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5
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1
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1
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1
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1
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5
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1
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1
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1
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2
1
1
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1
-
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740596
Somerville
Purification and characterizat ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
J. Bacteriol.
177
3837-3842
1995
1
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11
3
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2
4
-
4
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1
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1
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8
1
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3
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1
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3
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11
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3
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2
4
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1
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1
-
8
1
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-
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-
739974
Rieble
Aromatic nitroreductase from t ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
205
298-304
1994
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
2
-
7
-
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1
-
-
-
1
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-
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-
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-
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-
-
-
-
-
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-
1
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-
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-
-
1
-
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2
-
7
-
1
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1
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