BRENDA - Enzyme Database
show all sequences of 1.7.1.16

Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1

Wu, J.F.; Jiang, C.Y.; Wang, B.J.; Ma, Y.F.; Liu, Z.P.; Liu, S.J.; Appl. Environ. Microbiol. 72, 1759-1765 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Comamonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Comamonas sp.
Q5XW77
-
-
Comamonas sp. Ctestosteroni
Q5XW77
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-chloronitrobenzene + 2 NADPH + 2 H+
-
677659
Comamonas sp.
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
-
677659
Comamonas sp. Ctestosteroni
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
cnbA
-
Comamonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Comamonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Comamonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Comamonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-chloronitrobenzene + 2 NADPH + 2 H+
-
677659
Comamonas sp.
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
-
677659
Comamonas sp. Ctestosteroni
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
CnbA, CnbB, CnbCab, and CnbD genes encode chloronitrobenzene nitroreductase, hydroxylaminobenzene mutase, 2-aminophenol 1,6-dioxygenase, and 2-aminomuconic semialdehyde dehydrogenase, respectively. When these enzymes are coupled in vitro, they sequentially catalyze the conversions of 4-chloronitrobenzene to 2-amino-5-chloromuconic acid and nitrobenzene to 2-aminomuconic acid
Comamonas sp.
General Information (protein specific)
General Information
Commentary
Organism
physiological function
CnbA, CnbB, CnbCab, and CnbD genes encode chloronitrobenzene nitroreductase, hydroxylaminobenzene mutase, 2-aminophenol 1,6-dioxygenase, and 2-aminomuconic semialdehyde dehydrogenase, respectively. When these enzymes are coupled in vitro, they sequentially catalyze the conversions of 4-chloronitrobenzene to 2-amino-5-chloromuconic acid and nitrobenzene to 2-aminomuconic acid
Comamonas sp.
Other publictions for EC 1.7.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740989
Yang
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428-440
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740192
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In vivo imaging and detection ...
Danio rerio
Biosens. Bioelectron.
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112-116
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740325
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-
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Pseudomonas sp., Pseudomonas sp. N1
Chin. J. Appl. Environ. Biol.
19
1031-1034
2013
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741380
Isayev
In silico structure-function a ...
Enterobacter cloacae
Proteins
80
2728-2741
2012
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1
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741093
Yanto
Nitroreductase from Salmonella ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Org. Biomol. Chem.
8
1826-1832
2010
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6
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3
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1
1
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677659
Wu
Novel partial reductive pathwa ...
Comamonas sp., Comamonas sp. Ctestosteroni
Appl. Environ. Microbiol.
72
1759-1765
2006
-
-
1
-
-
-
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2
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677735
Xiao
Characterization of genes invo ...
Pseudomonas putida, Pseudomonas putida ZWL73
Appl. Microbiol. Biotechnol.
73
166-171
2006
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1
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7
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740133
Berne
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Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
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7
2631-2636
2006
-
1
1
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6
-
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4
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1
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6
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740929
Ho
-
Characterization of nitroreduc ...
Pseudomonas sp. HK-6
Korean J. Microbiol. Biotechnol.
32
230-237
2004
-
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5
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1
1
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740596
Somerville
Purification and characterizat ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
J. Bacteriol.
177
3837-3842
1995
1
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11
3
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2
4
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4
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1
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1
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739974
Rieble
Aromatic nitroreductase from t ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
205
298-304
1994
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