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Literature summary for 1.7.1.15 extracted from
- Heterologous expression and biochemical characterization of assimilatory nitrate and nitrite reductase reveals adaption and potential of Bacillus megaterium NCT-2 in secondary salinization soil (2017), Int. J. Biol. Macromol., 101, 1019-1028 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Priestia megaterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 1 mM, 84% of initial activity | Priestia megaterium |  |
| Ca2+ | 1 mM, 80% of initial activity | Priestia megaterium | |
| Cu2+ | 1 mM, 54% initial activity | Priestia megaterium | |
| Mg2+ | 1 mM, 82% of initial activity | Priestia megaterium | |
| additional information | not inhibitory nor activating: Ba2+, EDTA | Priestia megaterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
nitrite | pH 7.1, 30°C | Priestia megaterium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 1 mM, 226% of initial activity | Priestia megaterium | |
| Mn2+ | 1 mM, 110% of initial activity | Priestia megaterium | |
| additional information | not inhibitory nor activating: Ba2+, EDTA | Priestia megaterium | |
| Zn2+ | 1 mM, 113% of initial activity | Priestia megaterium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | A0A8D4BLJ9 and A0A8D4BRF4 | A0A8D4BLJ9 i.e subunit NasE, A0A8D4BRF4 i.e. electron transfer subunit NasB | - |
| Priestia megaterium WSH-002 | A0A8D4BLJ9 and A0A8D4BRF4 | A0A8D4BLJ9 i.e subunit NasE, A0A8D4BRF4 i.e. electron transfer subunit NasB | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrite + NADH + H+ | - |
Priestia megaterium | nitrite + NAD+ + H2O | - |
? | |
| nitrite + NADH + H+ | - |
Priestia megaterium WSH-002 | nitrite + NAD+ + H2O | - |
? | |
| nitrite + reduced methyl viologen | the optimum electron donor is methyl viologen plus Na2S2O4 | Priestia megaterium | ammonia + oxidized methyl viologen | - |
? | |
| nitrite + reduced methyl viologen | the optimum electron donor is methyl viologen plus Na2S2O4 | Priestia megaterium WSH-002 | ammonia + oxidized methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 87336, electron transfer subunit, x * 80100 catalytic subunit, SDS-PAGE | Priestia megaterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BMWSH_4089 | - |
Priestia megaterium |
| NasB | - |
Priestia megaterium |
| nasE | - |
Priestia megaterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Priestia megaterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
95% of maximum activity | Priestia megaterium |
| 80 | - |
50% of maximum activity | Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Priestia megaterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
95% of maximum activity | Priestia megaterium |
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