Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.15 extracted from

  • Jackson, R.H.; Cole, J.A.; Cornish-Bowden, A.
    The steady state kinetics of the NADH-dependent nitrite reductase from Escherichia coli K12. The reduction of single-electron acceptors (1982), Biochem. J., 203, 505-510.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NAD+ at 0.5 mM NAD+, the apparent maximum velocity is 2.3times higher for 0.1 mM cytochrome c as substrate than for 100 mM hydroxylamine Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
nitrite nitrite decrease the rate of NADH-dependent reduction of cytochrome c by 77% and that of Fe(CN)63- by 90% Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
cytochrome c apparent value, at pH 8.0 and 30°C Escherichia coli
0.011
-
nitrite apparent value, at pH 8.0 and 30°C Escherichia coli
0.0488
-
NADH with cytochrome c as cosubstrate, apparent value, at pH 8.0 and 30°C Escherichia coli
0.1
-
K3Fe(CN)6 apparent value, at pH 8.0 and 30°C Escherichia coli
2.91
-
hydroxylamine apparent value, at pH 8.0 and 30°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrite + NADH + H+ Escherichia coli
-
ammonia + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADH
-
Escherichia coli 2 ferrocytochrome c + NAD+ + H+
-
?
ferricytochrome c + NADH + H+
-
Escherichia coli ferrocytochrome c + NAD+
-
r
hydroxylamine + NADH + H+
-
Escherichia coli ?
-
?
K3Fe(CN)6 + NADH + H+
-
Escherichia coli ?
-
?
nitrite + NADH + H+
-
Escherichia coli ammonia + NAD+ + H2O
-
?

Synonyms

Synonyms Comment Organism
EC 1.6.6.4 formerly Escherichia coli
NADH-dependent nitrite reductase
-
Escherichia coli
NADH:nitrite oxidoreductase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH
-
Escherichia coli