Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 68% inhibition at 2 mM | Rhodobacter capsulatus | |
azide | 36% inhibition at 2 mM | Rhodobacter capsulatus | |
Cyanate | 27% inhibition at 2 mM | Rhodobacter capsulatus | |
cyanide | 69% inhibition at 2 mM | Rhodobacter capsulatus | |
dithioerythritol | 52% inhibition at 2 mM | Rhodobacter capsulatus | |
additional information | the activity of the His6-tagged NasB protein is unaffected by thiocyanate | Rhodobacter capsulatus | |
o-phenanthroline | 72% inhibition at 2 mM | Rhodobacter capsulatus | |
p-hydroxymercurybenzoate | 48% inhibition at 2 mM | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADH | at pH 9.0 and 30°C | Rhodobacter capsulatus | |
0.5 | - |
nitrite | at pH 9.0 and 30°C | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 2.8 mol iron per mol of protein | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86600 | - |
x * 86600, calculated from amino acid sequence | Rhodobacter capsulatus |
90000 | - |
x * 90000, His6-tagged enzyme, SDS-PAGE | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + NADH + H+ | Rhodobacter capsulatus | NADH is the best acceptor | ammonia + NAD+ + H2O | - |
? | |
nitrite + NADH + H+ | Rhodobacter capsulatus E1F1 | NADH is the best acceptor | ammonia + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | Q6WRS9 | - |
- |
Rhodobacter capsulatus E1F1 | Q6WRS9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
purified enzyme, at pH 9.0 and 30°C | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NADPH is not an effcient electron donor | Rhodobacter capsulatus | ? | - |
? | |
additional information | NADPH is not an effcient electron donor | Rhodobacter capsulatus E1F1 | ? | - |
? | |
nitrite + NADH + H+ | NADH is the best acceptor | Rhodobacter capsulatus | ammonia + NAD+ + H2O | - |
? | |
nitrite + NADH + H+ | NADH is the best acceptor | Rhodobacter capsulatus E1F1 | ammonia + NAD+ + H2O | - |
? | |
nitrite + reduced benzyl viologen | - |
Rhodobacter capsulatus | ammonia + oxidized benzyl viologen | - |
? | |
nitrite + reduced benzyl viologen | - |
Rhodobacter capsulatus E1F1 | ammonia + oxidized benzyl viologen | - |
? | |
nitrite + reduced methyl viologen | - |
Rhodobacter capsulatus | ammonia + oxidized methyl viologen | - |
? | |
nitrite + reduced methyl viologen | - |
Rhodobacter capsulatus E1F1 | ammonia + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 86600, calculated from amino acid sequence | Rhodobacter capsulatus |
? | x * 90000, His6-tagged enzyme, SDS-PAGE | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
assimilatory NADH-nitrite reductase | - |
Rhodobacter capsulatus |
NasB protein | - |
Rhodobacter capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodobacter capsulatus | |
Fe-S center | - |
Rhodobacter capsulatus | |
siroheme | - |
Rhodobacter capsulatus |