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Literature summary for 1.7.1.14 extracted from

  • Gong, B.; Liang, X.; Li, Y.; Xiao, Q.; Yang, P.; Wu, Y.
    Expression and purification of cytochrome P450 55B1 from Chlamydomonas reinhardtii and its application in nitric oxide biosensing (2018), Appl. Biochem. Biotechnol., 184, 102-112 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 NO + NADH + H+ Chlamydomonas reinhardtii
-
 N2O + NAD+ + H2O
-
 ?
2 NO + NADPH + H+ Chlamydomonas reinhardtii
-
 N2O + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography, and Superdex 200 gel filtration Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 NO + NADH + H+
-
 Chlamydomonas reinhardtii N2O + NAD+ + H2O
-
 ?
2 NO + NADPH + H+
-
 Chlamydomonas reinhardtii N2O + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
CYP55B1
-
 Chlamydomonas reinhardtii
cytochrome P450 55B1
-
 Chlamydomonas reinhardtii