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Literature summary for 1.7.1.13 extracted from

  • Jung, J.; Nidetzky, B.
    Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli (2018), J. Biol. Chem., 293, 3720-3733 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
E89A the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product Escherichia coli
E89L the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product Escherichia coli
F228A the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product Escherichia coli
I192A the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 1:1 ratio Escherichia coli
L191A the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 4:1 ratio Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-cyano-7-deazaguanine + 2 NADPH + 2 H+ Escherichia coli i.e. preQ0 7-aminomethyl-7-deazaguanine + 2 NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli Q46920
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-cyano-7-deazaguanine + 2 NADPH + 2 H+ i.e. preQ0 Escherichia coli 7-aminomethyl-7-deazaguanine + 2 NADP+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 71772, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
QueF
-
 Escherichia coli