Literature summary for 1.7.1.13 extracted from

Li, M.; Zhou, Z.; Zhang, Z.; Yu, H.; Xu, J.
Biochemical properties of a new nitrile reductase cloned from Pectobacterium carotovorum (2016), J. Mol. Catal. B, 131, 47-54 .

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pectobacterium carotovorum subsp. carotovorum

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology model based on the crystal structure of nitrile reductase from Vibrio cholerae, PDB ID 3uxv. The side chain of residue Glu89 and the main chain of Ser90 form hydrogen bonds with the ring nitrogen atoms of the substrate. The carboxylate group of Glu230 also forms hydrogen bonds with the substrate preQ0. The aromatic ring of Phe228 may form a pi-pi-stacking interaction with the aromatic ring of preQ0, while the main chain of His229coordinates the keto-functionality of the substrate	Pectobacterium carotovorum subsp. carotovorum

Crystallization (Comment)

Organism

homology model based on the crystal structure of nitrile reductase from Vibrio cholerae, PDB ID 3uxv. The side chain of residue Glu89 and the main chain of Ser90 form hydrogen bonds with the ring nitrogen atoms of the substrate. The carboxylate group of Glu230 also forms hydrogen bonds with the substrate preQ0. The aromatic ring of Phe228 may form a pi-pi-stacking interaction with the aromatic ring of preQ0, while the main chain of His229coordinates the keto-functionality of the substrate

Pectobacterium carotovorum subsp. carotovorum

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	0.1 mM, 89% residual activity	Pectobacterium carotovorum subsp. carotovorum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00025	-	7-cyano-7-carbaguanine	below 0.25 microM, pH 7.4, 30°C	Pectobacterium carotovorum subsp. carotovorum
0.229	-	NADPH	pH 7.4, 30°C	Pectobacterium carotovorum subsp. carotovorum

Metals/Ions

Metals/Ions	Comment	Organism
Cu2+	0.01 mM, 82% residual activity	Pectobacterium carotovorum subsp. carotovorum
Fe3+	0.01 mM, 92% residual activity	Pectobacterium carotovorum subsp. carotovorum
Ni2+	0.01 mM, 77% residual activity	Pectobacterium carotovorum subsp. carotovorum
Zn2+	0.01 mM, 13.5% residual activity	Pectobacterium carotovorum subsp. carotovorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62000	-	gel filtration	Pectobacterium carotovorum subsp. carotovorum

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium carotovorum subsp. carotovorum	C6DAH4	-	-
Pectobacterium carotovorum subsp. carotovorum PCC21	C6DAH4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
7-cyano-7-carbaguanine + 2 NADPH + 2 H+	-	Pectobacterium carotovorum subsp. carotovorum	7-aminomethyl-7-carbaguanine + 2 NADP+	-	?
7-cyano-7-carbaguanine + 2 NADPH + 2 H+	-	Pectobacterium carotovorum subsp. carotovorum PCC21	7-aminomethyl-7-carbaguanine + 2 NADP+	-	?
additional information	enzyme is highly specific for substrate preQ0	Pectobacterium carotovorum subsp. carotovorum	?	-	?
additional information	enzyme is highly specific for substrate preQ0	Pectobacterium carotovorum subsp. carotovorum PCC21	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 32000, SDS-PAGE	Pectobacterium carotovorum subsp. carotovorum

Synonyms

Synonyms	Comment	Organism
nitrile reductase	-	Pectobacterium carotovorum subsp. carotovorum
NRed	-	Pectobacterium carotovorum subsp. carotovorum
QueF	-	Pectobacterium carotovorum subsp. carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Pectobacterium carotovorum subsp. carotovorum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
50	-	more than 90% of maximum activity	Pectobacterium carotovorum subsp. carotovorum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	half-life 47.2 h	Pectobacterium carotovorum subsp. carotovorum
40	-	half-life 13.6 h	Pectobacterium carotovorum subsp. carotovorum
50	-	half-life 3.9 h	Pectobacterium carotovorum subsp. carotovorum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.024	-	7-cyano-7-carbaguanine	pH 7.4, 30°C	Pectobacterium carotovorum subsp. carotovorum
0.025	-	NADPH	pH 7.4, 30°C	Pectobacterium carotovorum subsp. carotovorum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	-	Pectobacterium carotovorum subsp. carotovorum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8	more than 60% of maximum activity	Pectobacterium carotovorum subsp. carotovorum

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Pectobacterium carotovorum subsp. carotovorum