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Literature summary for 1.7.1.13 extracted from

  • Moeller, K.; Nguyen, G.S.; Hollmann, F.; Hanefeld, U.
    Expression and characterization of the nitrile reductase queF from E. coli (2013), Enzyme Microb. Technol., 52, 129-133.
    View publication on PubMed
Search for more literature for 1.7.1.13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 NADPH or below, pH 7.0, 37°C Escherichia coli
0.0015
-
 7-cyano-7-deazaguanine or below, pH 7.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q46920
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-cyano-7-deazaguanine + NADPH + H+
-
 Escherichia coli queuine + NADP+ + H+
-
 ?
additional information no substrates: acetonitrile, benzonitrile and benzylcyanide Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
QueF
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 half-life 28.2 Escherichia coli
40
-
 half-life 12.8 Escherichia coli
45
-
 half-life 2.8 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0033
-
 NADPH pH 7.0, 37°C Escherichia coli
0.127
-
 7-cyano-7-deazaguanine pH 7.0, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 Tris-buffer Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 4 h, no loss of activity Escherichia coli
9
-
 4 h, 80% residual activity Escherichia coli