BRENDA - Enzyme Database
show all sequences of 1.7.1.12

Purification of N-hydroxy-2-acetylaminofluorene reductase from rabbit liver cytosol

Kitamura, S.; Tatsumi, K.; Biochem. Biophys. Res. Commun. 133, 67-74 (1985)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
enhances activity, can replace NADH
Oryctolagus cuniculus
cysteine
enhances activity, can replace NADH
Oryctolagus cuniculus
dithiothreitol
enhances activity, can replace NADH
Oryctolagus cuniculus
glutathione
enhances activity, can replace NADH
Oryctolagus cuniculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
1 mM, 52% inhibition
Oryctolagus cuniculus
Disulfiram
0.1 mM, 56% inhibition
Oryctolagus cuniculus
N-ethylmaleimide
1 mM, 86% inhibition
Oryctolagus cuniculus
p-chloromercuribenzoate
0.2 mM, 81% inhibition
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
microsome
low amounts
Oryctolagus cuniculus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
gel filtration
Oryctolagus cuniculus
Organism
Organism
UniProt
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
910
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N-hydroxy-2-acetamidofluorene + NAD(P)H
other electron donors: cysteine, glutathione, dithiothreitol, 2-mercaptoethanol
394431
Oryctolagus cuniculus
2-acetamidofluorene + NAD(P)+ + H2O
-
394431
Oryctolagus cuniculus
?
N-hydroxy-4-acetylaminobiphenyl + NAD(P)H
much slower reaction compared to N-hydroxy-2-acetamidofluorene
394431
Oryctolagus cuniculus
4-acetylaminobiphenyl + NAD(P)+ + H2O
-
394431
Oryctolagus cuniculus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 34000, SDS-PAGE
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Oryctolagus cuniculus
NADPH
-
Oryctolagus cuniculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
enhances activity, can replace NADH
Oryctolagus cuniculus
cysteine
enhances activity, can replace NADH
Oryctolagus cuniculus
dithiothreitol
enhances activity, can replace NADH
Oryctolagus cuniculus
glutathione
enhances activity, can replace NADH
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Oryctolagus cuniculus
NADPH
-
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
1 mM, 52% inhibition
Oryctolagus cuniculus
Disulfiram
0.1 mM, 56% inhibition
Oryctolagus cuniculus
N-ethylmaleimide
1 mM, 86% inhibition
Oryctolagus cuniculus
p-chloromercuribenzoate
0.2 mM, 81% inhibition
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
microsome
low amounts
Oryctolagus cuniculus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
gel filtration
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
910
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N-hydroxy-2-acetamidofluorene + NAD(P)H
other electron donors: cysteine, glutathione, dithiothreitol, 2-mercaptoethanol
394431
Oryctolagus cuniculus
2-acetamidofluorene + NAD(P)+ + H2O
-
394431
Oryctolagus cuniculus
?
N-hydroxy-4-acetylaminobiphenyl + NAD(P)H
much slower reaction compared to N-hydroxy-2-acetamidofluorene
394431
Oryctolagus cuniculus
4-acetylaminobiphenyl + NAD(P)+ + H2O
-
394431
Oryctolagus cuniculus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 34000, SDS-PAGE
Oryctolagus cuniculus
Other publictions for EC 1.7.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
394431
Kitamura
Purification of N-hydroxy-2-ac ...
Oryctolagus cuniculus
Biochem. Biophys. Res. Commun.
133
67-74
1985
4
-
-
-
-
-
4
-
3
-
1
-
-
3
-
-
1
-
-
2
1
-
2
1
-
-
-
-
-
-
-
-
2
-
-
-
4
-
-
2
-
-
-
-
4
-
-
3
-
1
-
-
-
-
1
-
2
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-