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Literature summary for 1.7.1.12 extracted from
- Purification of N-hydroxy-2-acetylaminofluorene reductase from rabbit liver cytosol (1985), Biochem. Biophys. Res. Commun., 133, 67-74.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | enhances activity, can replace NADH | Oryctolagus cuniculus |  |
| cysteine | enhances activity, can replace NADH | Oryctolagus cuniculus | |
| dithiothreitol | enhances activity, can replace NADH | Oryctolagus cuniculus | |
| glutathione | enhances activity, can replace NADH | Oryctolagus cuniculus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | 1 mM, 52% inhibition | Oryctolagus cuniculus | |
| Disulfiram | 0.1 mM, 56% inhibition | Oryctolagus cuniculus | |
| N-ethylmaleimide | 1 mM, 86% inhibition | Oryctolagus cuniculus | |
| p-chloromercuribenzoate | 0.2 mM, 81% inhibition | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
| microsome | low amounts | Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 910 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-hydroxy-2-acetamidofluorene + NAD(P)H | other electron donors: cysteine, glutathione, dithiothreitol, 2-mercaptoethanol | Oryctolagus cuniculus | 2-acetamidofluorene + NAD(P)+ + H2O | - |
? | |
| N-hydroxy-4-acetylaminobiphenyl + NAD(P)H | much slower reaction compared to N-hydroxy-2-acetamidofluorene | Oryctolagus cuniculus | 4-acetylaminobiphenyl + NAD(P)+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Oryctolagus cuniculus | |
| NADPH | - |
Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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