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Literature summary for 1.7.1.1 extracted from

  • Chow, F.; Pedersen, M.; Oliveira, M.
    Modulation of nitrate reductase activity by photosynthetic electron transport chain and nitric oxide balance in the red macroalga Gracilaria chilensis (Gracilariales, Rhodophyta) (2013), J. Appl. Phycol., 25, 1847-1853 .
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
8-bromo-cyclic GMP addition of 8Br-cGMP, an analogous compound of the secondary messenger cGMP and a secondary signal molecule itself, increases significantly nitrate reductase activity by 51% at 0.01 mM Agarophyton chilense
methylene blue a guanyl cyclase inhibitor, increases significantly the enzymatic activity by 141% at 0.05 mM Agarophyton chilense

Inhibitors

Inhibitors Comment Organism Structure
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone a photosynthetic electron flux inhibitor of photosystem I, causes 61% inhibition at 0.008 mM Agarophyton chilense
2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide a NO scavenger Agarophyton chilense
3-(3,4-dichlorophenyl)-1,1-dimethylurea a photosynthetic electron flux inhibitor of photosystem II, causes 51% inhibition at 0.001 mM Agarophyton chilense
additional information no enzyme inhibition by diphenyleneiodonium, i.e. DPI, a NADPH oxidase inhibitor, and poor inhibition by L-NG-monomethyl arginine citrate (L-NMMA), a NOS inhibitor. But nitrate reductase activity is sensitive to a decrease or increase of NO levels when NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and NO donor sodium nitroprusside are added Agarophyton chilense
sodium nitroprusside a NO donor Agarophyton chilense

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Agarophyton chilense 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum
-
 Agarophyton chilense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADH + H+ Agarophyton chilense
-
 nitrite + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Agarophyton chilense
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADH + H+
-
 Agarophyton chilense nitrite + NAD+
-
 r

Subunits

Subunits Comment Organism
homodimer
-
 Agarophyton chilense

Synonyms

Synonyms Comment Organism
nitrate reductase
-
 Agarophyton chilense

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Agarophyton chilense

General Information

General Information Comment Organism
metabolism nitric oxide is an important signaling molecule regulating nitrate reductase activity, and cGMP participates as secondary messenger on this regulation by phosphorylation and desphosphorylation processes Agarophyton chilense
physiological function nitrate reductase is the first enzyme in the nitrogen assimilatory pathway, which reduces nitrate to nitrite. Nitrate reductase is a key enzyme in nitrogen metabolism, that has been implicated in the production of nitric oxide (NO) in plants. In the red macroalga, nitrate reductase activity is modulated by photosynthetic electron transport chain and nitric oxide balance, direct dependence of nitrate reductase activity on the PSII and PSI electron flux, overview Agarophyton chilense