Cloned (Comment) | Organism |
---|---|
expressed in Nicotiana plumbaginifolia | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
S521D | the protein is constitutively active under conditions where the wild type protein would be rapidly inactivated, such as in the dark | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
14-3-3 protein BMH1 | 0.0018 mM, reduces activity to less than 20%, at pH 6.0 | Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Ble Sepharose chromatography | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADH | - |
Nicotiana tabacum | nitrite + NAD+ + H2O | - |
? | |
nitrite + NADH | - |
Nicotiana tabacum | nitric oxide + NAD+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Nicotiana tabacum |