Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | at low pH, the presence of ATP alone in the incubation medium is sufficient to inactivate nitrate reductase | Cucumis sativus | |
Mg2+ | 5 mM MgCl2 decreases enzyme activity at pH 7.5, this effect is completely reversed by the addition of EDTA into samples returning the enzyme's activity to its initial level | Cucumis sativus | |
additional information | An inhibitory effect of Mg-ATP is cancelled in the presence of staurosporine (the protein kinase inhibitor) and completely reversed after addition of EDTA as well as AMP | Cucumis sativus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 27% increase of activity at pH 7.5 | Cucumis sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucumis sativus | - |
cultivar Wisconsin | - |
Purification (Comment) | Organism |
---|---|
Sephadex G-25 gel filtration | Cucumis sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Cucumis sativus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADH | - |
Cucumis sativus | nitrite + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADH-dependent nitrate reductase | - |
Cucumis sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Cucumis sativus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
activity determined at pH 6.0 was significantly lower than at pH 7.5 | Cucumis sativus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cucumis sativus |