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Literature summary for 1.7.1.1 extracted from
- Bacterial expression of the molybdenum domain of assimilatory nitrate reductase: production of both the functional molybdenum-containing domain and the nonfunctional tungsten analog (2002), Arch. Biochem. Biophys., 403, 237-248.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of amino-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein | Spinacia oleracea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of 541 residue amonio-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein | Spinacia oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
nitrate | His-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea |  |
| 0.012 | - |
nitrate | GST-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | Mo/protein ratio 0.44 for His-tagged protein, 0.93 for GST-tagged protein | Spinacia oleracea | |
| additional information | tungsten cannot replace for molybdenum in the GST-tagged protein | Spinacia oleracea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain | Spinacia oleracea |
| 85000 | - |
x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
expression of amino-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0015 | - |
His-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea |
| 0.0108 | - |
GST-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced methyl viologen + nitrate | - |
Spinacia oleracea | methyl viologen + nitrite | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain | Spinacia oleracea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
nitrate | GST-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea | |
| 2.5 | - |
nitrate | His-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea | |
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