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Literature summary for 1.7.1.1 extracted from
- The assimilatory nitrate reductase from the phototrophic bacterium, Rhodobacter capsulatus E1F1, is a flavoprotein (1997), FEBS Lett., 414, 45-49.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.052 | - |
NADH | reaction with nitrate | Rhodobacter capsulatus |  |
| 0.067 | - |
NADH | reaction with 2,6-dichlorophenol indophenol | Rhodobacter capsulatus | |
| 0.096 | - |
nitrate | reaction with NADH | Rhodobacter capsulatus | |
| 0.13 | - |
nitrate | reaction with methyl viologen | Rhodobacter capsulatus | |
| 0.54 | - |
reduced bromophenol blue | - |
Rhodobacter capsulatus | |
| 0.87 | - |
reduced methyl viologen | - |
Rhodobacter capsulatus | |
| 13 | - |
nitrate | reaction with bromophenol blue | Rhodobacter capsulatus | |
| 80 | - |
ferricytochrome c | - |
Rhodobacter capsulatus | |
| 100 | - |
oxidized 2,6-dichlorophenolindophenol | - |
Rhodobacter capsulatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
1 * 46000 + 1 * 95000, SDS-PAGE | Rhodobacter capsulatus |
| 95000 | - |
1 * 46000 + 1 * 95000, SDS-PAGE | Rhodobacter capsulatus |
| 144000 | - |
non-denaturing pore gradient PAGE | Rhodobacter capsulatus |
| 147000 | - |
gel filtration | Rhodobacter capsulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | - |
E1F1 | - |
| Rhodobacter capsulatus E1F1 | - |
E1F1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodobacter capsulatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADH | - |
Rhodobacter capsulatus | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
| 2 ferricytochrome c + NADH | - |
Rhodobacter capsulatus E1F1 | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
| nitrate + NADH | - |
Rhodobacter capsulatus | nitrite + NAD+ + H2O | - |
? | |
| nitrate + NADH | - |
Rhodobacter capsulatus E1F1 | nitrite + NAD+ + H2O | - |
? | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Rhodobacter capsulatus | ? + NAD+ | - |
? | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Rhodobacter capsulatus E1F1 | ? + NAD+ | - |
? | |
| oxidized 2,6-dichlorophenolindophenol + NADH + H+ | - |
Rhodobacter capsulatus | reduced 2,6-dichlorophenolindophenol + NAD+ | - |
r | |
| reduced bromophenol blue + nitrate | - |
Rhodobacter capsulatus | bromophenol blue + nitrite | - |
? | |
| reduced bromophenol blue + nitrate | - |
Rhodobacter capsulatus E1F1 | bromophenol blue + nitrite | - |
? | |
| reduced methyl viologen + nitrate | - |
Rhodobacter capsulatus | methyl viologen + nitrite | - |
? | |
| reduced methyl viologen + nitrate | - |
Rhodobacter capsulatus E1F1 | methyl viologen + nitrite | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 46000 + 1 * 95000, SDS-PAGE | Rhodobacter capsulatus |
| More | diaphorase activity is located in the small subunit | Rhodobacter capsulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
NADH-diaphorase activity | Rhodobacter capsulatus |
| 9 | - |
NADH-nitrate reductase activity | Rhodobacter capsulatus |
| 9.5 | - |
methyl viologen-nitrate reductase activity | Rhodobacter capsulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Rhodobacter capsulatus | |
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