BRENDA - Enzyme Database show
show all sequences of 1.6.99.3

NADH dehydrogenase from bovine neutrophil membranes. Purification and properties

Nisimoto, Y.; Wilson, E.; Heyl, B.L.; Lambeth, J.D.; J. Biol. Chem. 261, 285-290 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
NAD+
weakly competitive
Bos taurus
NEM
0.5 mM, 12% inhibition
Bos taurus
PCMB
0.005 mM, 50% inhibition
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0095
-
ferricyanide
-
Bos taurus
0.018
-
NADH
-
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
associated
Bos taurus
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17500
-
x * 17500, SDS-PAGE
Bos taurus
480000
-
highly aggregated enzyme form, non-denaturing PAGE
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
neutrophil
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bos taurus
Storage Stability
Storage Stability
Organism
-20°C, stable for several months
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 ferricyanide + NADH
-
392819
Bos taurus
2 ferrocyanide + NAD+ + H+
-
-
-
?
2 ferricytochrome b5 + NADH
-
392819
Bos taurus
2 ferrocytochrome b5 + NAD+ + H+
-
-
-
?
2 ferricytochrome c + NADH
-
392819
Bos taurus
2 ferrocytochrome c + NAD+ + H+
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
-
392819
Bos taurus
NAD+ + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 17500, SDS-PAGE
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no flavin detectable
Bos taurus
NADH
-
Bos taurus
NADPH
-
Bos taurus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.118
-
NAD+
-
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no flavin detectable
Bos taurus
NADH
-
Bos taurus
NADPH
-
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NAD+
weakly competitive
Bos taurus
NEM
0.5 mM, 12% inhibition
Bos taurus
PCMB
0.005 mM, 50% inhibition
Bos taurus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.118
-
NAD+
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0095
-
ferricyanide
-
Bos taurus
0.018
-
NADH
-
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
associated
Bos taurus
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17500
-
x * 17500, SDS-PAGE
Bos taurus
480000
-
highly aggregated enzyme form, non-denaturing PAGE
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
neutrophil
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for several months
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 ferricyanide + NADH
-
392819
Bos taurus
2 ferrocyanide + NAD+ + H+
-
-
-
?
2 ferricytochrome b5 + NADH
-
392819
Bos taurus
2 ferrocytochrome b5 + NAD+ + H+
-
-
-
?
2 ferricytochrome c + NADH
-
392819
Bos taurus
2 ferrocytochrome c + NAD+ + H+
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
-
392819
Bos taurus
NAD+ + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 17500, SDS-PAGE
Bos taurus
Other publictions for EC 1.6.99.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741964
Villegas
FAD binding properties of a c ...
Escherichia coli
Biochim. Biophys. Acta
1844
576-584
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724488
Villegas
Maintenance and thermal stabil ...
Arabidopsis thaliana, Marchantia polymorpha, Nicotiana tabacum, Synechocystis sp., Zea mays
Biochimie
95
382-387
2013
-
-
-
-
-
-
-
-
8
-
-
-
-
5
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
14
14
-
-
-
746936
Lakhal
Oxygen uptake rates in the hy ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Arch. Microbiol.
193
429-438
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
701758
Do
Engineering Escherichia coli f ...
Trichomonas vaginalis
Appl. Biochem. Biotechnol.
153
21-33
2009
-
-
1
-
-
-
-
6
-
-
3
-
-
7
-
-
1
-
-
-
4
-
4
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
3
-
-
-
-
1
-
-
4
-
4
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
685693
Chakraborty
Two proteins with diaphorase a ...
Moorella thermoacetica
Biosci. Biotechnol. Biochem.
72
877-879
2008
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
692853
Platt
Proteomic, microarray, and sig ...
Pseudomonas aeruginosa
J. Bacteriol.
190
2739-2758
2008
-
-
1
-
-
-
1
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677088
Kitazume
Involvement of Lys-308 in the ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) YN-1
Res. Microbiol.
157
956-959
2006
-
-
-
-
2
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658494
Shiraki
Involvement of glycine and asp ...
Bacillus sp. YN-1
Curr. Microbiol.
46
432-434
2003
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392824
Marques
Activation of a NADH dehydroge ...
Homo sapiens
Biol. Signals
6
52-61
1997
1
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392825
Thiagalingam
-
Purification and characterizat ...
Bacillus megaterium
Can. J. Microbiol.
39
826-833
1993
-
-
-
-
-
-
10
4
1
-
1
1
-
1
-
-
1
-
-
-
1
-
5
1
-
-
-
-
1
-
-
2
1
-
-
-
-
-
2
-
-
-
-
10
1
4
1
-
1
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392819
Nisimoto
NADH dehydrogenase from bovine ...
Bos taurus
J. Biol. Chem.
261
285-290
1986
-
-
-
-
-
-
3
2
2
-
2
-
-
3
-
-
1
-
-
1
1
1
4
1
-
-
-
-
-
-
-
3
1
-
-
-
-
-
3
-
-
-
-
3
1
2
2
-
2
-
-
-
-
1
-
1
1
1
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392812
Borneleit
-
Purification and properties of ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
722
94-101
1983
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392820
Bergsma
Purification and characterizat ...
Bacillus subtilis, Bacillus subtilis W23
Eur. J. Biochem.
128
151-157
1982
-
-
-
-
-
-
3
-
4
-
1
-
-
8
-
-
-
-
-
1
1
-
4
1
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
-
4
-
1
-
-
-
-
-
-
1
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
392811
Kitajima
Purification and properties of ...
Homo sapiens
Arch. Biochem. Biophys.
210
330-339
1981
-
-
-
-
-
-
7
2
1
-
2
-
-
1
-
-
1
-
-
1
1
-
6
1
-
-
-
1
2
-
-
3
1
-
-
-
-
-
3
-
-
-
-
7
1
2
1
-
2
-
-
-
-
1
-
1
1
-
6
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
392810
Wang
Human erythrocyte NADH: (accep ...
Homo sapiens
Biochim. Biophys. Acta
616
22-29
1980
-
-
-
-
-
-
3
2
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392805
Adachi
Study on the reduced pyridine ...
Bos taurus
Biochim. Biophys. Acta
268
629-637
1972
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392821
Ramanarayanan
Purification & properties of a ...
Agrobacterium tumefaciens
Indian J. Biochem. Biophys.
8
214-218
1971
-
-
-
-
-
-
1
1
1
-
-
-
-
1
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
1
-
1
1
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392807
Kaniuga
The Transformation of mitochon ...
Sus scrofa
Biochim. Biophys. Acta
73
550-564
1963
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-