Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.6.5.9 extracted from

  • Cook, N.; Cammack, R.
    Purification and characterization of the rotenone-insensitive NADH dehydrogenase of mitochondria from Arum maculatum (1984), Eur. J. Biochem., 141, 573-577.
    View publication on PubMed
Search for more literature for 1.6.5.9

Inhibitors

Inhibitors Comment Organism Structure
flavone
-
 Arum maculatum
additional information the enzyme is not inhibited by rotenone, and activity is not affected by EDTA (1 mM), EGTA (1 mM) or Ca2+ or Mg2+ (1 mM) Arum maculatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 NADH at 25°C and pH 7.2 Arum maculatum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Arum maculatum 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33900
-
 the rotenone-insensitive NADH-ubiquinone-1 reductase activity eluted by 0.5 M NaCl displays three major bands on SDS-PAGE of 55000, 39200 and 33900 Da Arum maculatum
39200
-
 the rotenone-insensitive NADH-ubiquinone-1 reductase activity eluted by 0.5 M NaCl displays three major bands on SDS-PAGE of 55000, 39200 and 33900 Da Arum maculatum
55000
-
 the rotenone-insensitive NADH-ubiquinone-1 reductase activity eluted by 0.5 M NaCl displays three major bands on SDS-PAGE of 55000, 39200 and 33900 Da Arum maculatum

Organism

Organism UniProt Comment Textmining
Arum maculatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
5'-ADP-Sepharose 4B column chromatography Arum maculatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7
-
 crude enzyme, using ubiquinone-1 as substrate, at 25°C and pH 7.2 Arum maculatum
69.2
-
 after 18.7fold purification, using ubiquinone-1 as substrate, at 25°C and pH 7.2 Arum maculatum
3372
-
 crude enzyme, using ubiquinone-1 as substrate, at 25°C and pH 7.2 Arum maculatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH 15% activity compared to ubiquinone-1 Arum maculatum 2 ferrocyanide + NAD+ + H+
-
 ?
additional information no activity with cytochrome c Arum maculatum ?
-
 ?
NADH + H+ + 2,6-dichloroindophenol 17% activity compared to ubiquinone-1 Arum maculatum NAD+ + reduced 2,6-dichloroindophenol
-
 ?
NADH + H+ + duroquinone 49% activity compared to ubiquinone-1 Arum maculatum NAD+ + duroquinol
-
 ?
NADH + H+ + juglone 29% activity compared to ubiquinone-1 Arum maculatum NAD+ + ?
-
 ?
NADH + H+ + menadione 47% activity compared to ubiquinone-1 Arum maculatum NAD+ + menadiol
-
 ?
NADH + H+ + ubiquinone-1 100% activity Arum maculatum NAD+ + ubiquinol-1
-
 ?

Synonyms

Synonyms Comment Organism
rotenone-insensitive NADH dehydrogenase
-
 Arum maculatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 pH-optimum around pH 7.2 Arum maculatum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Arum maculatum
additional information no iron-sulfur centers Arum maculatum
NADH the partially purified enzyme is specific for NADH above pH 7.0 Arum maculatum
NADPH below pH 7.0 NADPH is slowly oxidized Arum maculatum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.175
-
 at 25°C and pH 7.2 Arum maculatum flavone