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Literature summary for 1.6.5.4 extracted from

  • Negi, B.; Salvi, P.; Bhatt, D.; Majee, M.; Arora, S.
    Molecular cloning, in-silico characterization and functional validation of monodehydroascorbate reductase gene in Eleusine coracana (2017), PLoS ONE, 12, e0187793 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.5.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Eleusine coracana

Crystallization (Commentary)

Crystallization (Comment) Organism
3D model of Mdar protein contains 21 sheets and 15 helices and 33 loop regions. The active site is comprised of residues Glu39, Glu40, Glu46, Arg47, Pro48, Lys52, Thr122, Asn147, Tyr173, Pro194, Glu195, Arg201, Ile261, Asn264, Asp299, Glu316, His317, Arg322, Phe347 and Tyr348 Eleusine coracana

Organism

Organism UniProt Comment Textmining
Eleusine coracana A0A0U2UAN2
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-

Subunits

Subunits Comment Organism
? x * 52080, calculated from sequence, x * 52000, SDS-PAGE Eleusine coracana

Synonyms

Synonyms Comment Organism
MDAR
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 Eleusine coracana

Cofactor

Cofactor Comment Organism Structure
FAD the conserved NAD and FAD binding domains contain conserved Cys69, Phe352, Tyr173, Tyr348 and His317 residues Eleusine coracana
NADH the conserved NAD and FAD binding domains contain conserved Cys69, Phe352, Tyr173, Tyr348 and His317 residues Eleusine coracana

pI Value

Organism Comment pI Value Maximum pI Value
Eleusine coracana calculated from sequence
-
 9.1

Expression

Organism Comment Expression
Eleusine coracana mdar is an early responsive gene under stress additional information

General Information

General Information Comment Organism
physiological function monodehydroascorbate reductase acts as a key stress regulator by modulating the activity of antioxidant enzymes to strengthen the ROS scavenging ability and maintains ROS homeostasis. Arabidopsis thaliana transgenic plants expressing Mdar do not show any significant differences in the growth pattern and plant morphology. Under 100 mM NaCl stress, transgenic plants show better growth as compared to the wild-type plants Eleusine coracana