KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NADPH | pH 7.8, temperature not specified in the publication | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FUP0 | cf. EC 1.3.1.42 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADPH + H+ | - |
Arabidopsis thaliana | 2 ferrocyanide + NADP+ | - |
? | |
monodehydroascorbate + NADPH + H+ | - |
Arabidopsis thaliana | ascorbate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE, x * 42690, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
12-oxophytodienoate reductase 3 | - |
Arabidopsis thaliana |
OPR3 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | absorption maxima at 280, 374 and 467 nm | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | OPR3 is a bifunctional enzyme with NADPH-dependent alpha,beta-ketoalkene double-bond reductase and monodehydroascorbate reductase activities. OPR3 mutants show a slightly less-reduced ascorbate pool in leaves in line with the MDHAR activity of OPR3 in vitro. OPR3 catalyzes the reduction of a wide spectrum of electrophilic species that share a reactivity toward the glutathione and ascorbate. 12-oxophytodienoic acid reacts with ascorbate to form an ascorbate-12-oxophytodienoic acid adduct, but in addition OPR3 has the ability to regenerate ascorbate from monodehydroascorbate | Arabidopsis thaliana |