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Literature summary for 1.6.5.4 extracted from
- Tobacco Nectarin III is a bifunctional enzyme with monodehydroascorbate reductase and carbonic anhydrase activities (2004), Plant Mol. Biol., 54, 415-425.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Nicotiana langsdorffii x Nicotiana sanderae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | coordinated by residues His122, His124, and His141 | Nicotiana langsdorffii x Nicotiana sanderae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28536 | - |
x * 40000, SDS-PAGE, x * 28536, amino acid sequence calculation | Nicotiana langsdorffii x Nicotiana sanderae |
| 40000 | - |
x * 40000, SDS-PAGE, x * 28536, amino acid sequence calculation | Nicotiana langsdorffii x Nicotiana sanderae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + H2O | Nicotiana langsdorffii x Nicotiana sanderae | bifunctional enzyme showing monodehydroascorbate reductase and carbonic anhydrase, EC 4.2.1.1, activities | HCO3- + H+ | - |
? | |
| additional information | Nicotiana langsdorffii x Nicotiana sanderae | reaction scheme, the enzyme is involved in nectary production in floral organs, multiple molecular mechanism responsible for expression in the nectary gland | ? | - |
? | |
| NADH + monodehydroascorbate | Nicotiana langsdorffii x Nicotiana sanderae | enzyme is responsible for maintaining ascorbate concentrations in nectar | NAD+ + ascorbate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana langsdorffii x Nicotiana sanderae | Q84UV8 | interspecific cross of Nicotiana lansdorfii x Nicotiana sanderae | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| floral tube | lower expression level | Nicotiana langsdorffii x Nicotiana sanderae | - |
| flower bud | early to post-fertilized | Nicotiana langsdorffii x Nicotiana sanderae | - |
| additional information | detailed analysis of expression during nectary development in flower organs, overview | Nicotiana langsdorffii x Nicotiana sanderae | - |
| nectary | different developmental stages, high expression level | Nicotiana langsdorffii x Nicotiana sanderae | - |
| ovary | lower expression level | Nicotiana langsdorffii x Nicotiana sanderae | - |
| stigma | lower expression level | Nicotiana langsdorffii x Nicotiana sanderae | - |
| style | lower expression level | Nicotiana langsdorffii x Nicotiana sanderae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + H2O | bifunctional enzyme showing monodehydroascorbate reductase and carbonic anhydrase, EC 4.2.1.1, activities | Nicotiana langsdorffii x Nicotiana sanderae | HCO3- + H+ | - |
? | |
| additional information | reaction scheme, the enzyme is involved in nectary production in floral organs, multiple molecular mechanism responsible for expression in the nectary gland | Nicotiana langsdorffii x Nicotiana sanderae | ? | - |
? | |
| NADH + monodehydroascorbate | - |
Nicotiana langsdorffii x Nicotiana sanderae | NAD+ + ascorbate | - |
? | |
| NADH + monodehydroascorbate | enzyme is responsible for maintaining ascorbate concentrations in nectar | Nicotiana langsdorffii x Nicotiana sanderae | NAD+ + ascorbate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE, x * 28536, amino acid sequence calculation | Nicotiana langsdorffii x Nicotiana sanderae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme is bifunctional showing monodehydroascorbate reductase and carbonic anhydrase, EC 4.2.1.1, activities | Nicotiana langsdorffii x Nicotiana sanderae |
| NEC3 | - |
Nicotiana langsdorffii x Nicotiana sanderae |
| Nectarin III | - |
Nicotiana langsdorffii x Nicotiana sanderae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Nicotiana langsdorffii x Nicotiana sanderae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
stable pH of nectar in vivo | Nicotiana langsdorffii x Nicotiana sanderae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Nicotiana langsdorffii x Nicotiana sanderae | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Nicotiana langsdorffii x Nicotiana sanderae | amino acid sequence calculation | - |
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