Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Cucumis sativus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, immediately after purification by hanging drop vapour diffusion method, 0.005 ml of protein solution containing 10 mg/ml protein, mixed with equal volume of reservoir solution containing 20-25% PEG 6000, 0.1 M CaCl2, 50 mM Tris-HCl, pH 8.0, equilibration against 1 ml reservoir solution, 20°C, 1 week, cryoprotection by 30% glycerol, X-ray diffraction structure determination and analysis at 2.4 A resolution | Cucumis sativus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
thiol-modifiying reagents | inhibition at the step of FAD reduction by NADH producing a charge-transfer complex | Cucumis sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Cucumis sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47300 | - |
x * 47300, SDS-PAGE | Cucumis sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucumis sativus | - |
cucumber | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Cucumis sativus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NADH + H+ + 2 monodehydroascorbate = NAD+ + 2 ascorbate | ping-pong mechanism | Cucumis sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD(P)H + monodehydroascorbate | reduction of the radical monodehydroascorbate, via FAD as intermediary electron acceptor, FAD reduction by NADH producing a charge-transfer complex | Cucumis sativus | NAD(P)+ + ascorbate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47300, SDS-PAGE | Cucumis sativus |
Synonyms | Comment | Organism |
---|---|---|
MDA | - |
Cucumis sativus |
monodehydroascorbate radical reductase | - |
Cucumis sativus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Cucumis sativus | |
NAD(P)H | - |
Cucumis sativus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cucumis sativus | sequence calculation | - |
6 |