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Literature summary for 1.6.5.2 extracted from

  • Lienhart, W.D.; Strandback, E.; Gudipati, V.; Koch, K.; Binter, A.; Uhl, M.K.; Rantasa, D.M.; Bourgeois, B.; Madl, T.; Zangger, K.; Gruber, K.; Macheroux, P.
    Catalytic competence, structure and stability of the cancer-associated R139W variant of the human NAD(P)H quinone oxidoreductase 1 (NQO1) (2017), FEBS J., 284, 1233-1245 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.5.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant R139W, to 2.09 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
R139W single nucleotide polymorphism, variant protein adopts the same structure both in the crystal as in solution, and kinetic parameters are similar to those reported for the wild-type protein. Thermostability of the variant is only slightly affected Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0106
-
 menadione mutant R139W, pH 7.0,25°C Homo sapiens
0.0116
-
 menadione wild-type, pH 7.0,25°C Homo sapiens
3.22
-
 NADH wild-type, pH 7.0,25°C Homo sapiens
3.31
-
 NADH mutant R139W, pH 7.0,25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P15559
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
menadione + NADH + H+
-
 Homo sapiens reduced menadione + NAD+
-
 ?
menadione + NADPH + H+
-
 Homo sapiens reduced menadione + NADP+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 melting point, mutant R139W Homo sapiens
54
-
 melting point, wild-type Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1580
-
 NADH mutant R139W, pH 7.0,25°C Homo sapiens
1590
-
 NADH wild-type, pH 7.0,25°C Homo sapiens
1730
-
 menadione mutant R139W, pH 7.0,25°C Homo sapiens
1830
-
 menadione wild-type, pH 7.0,25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD absorption spectra of wild-type and mutant R139W with maxima at 375 and 450 nm Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
478
-
 NADH mutant R139W, pH 7.0,25°C Homo sapiens
494
-
 NADH wild-type, pH 7.0,25°C Homo sapiens
158000
-
 menadione wild-type, pH 7.0,25°C Homo sapiens
162000
-
 menadione mutant R139W, pH 7.0,25°C Homo sapiens