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Literature summary for 1.6.5.2 extracted from

  • Gonzalez, C.F.; Ackerley, D.F.; Lynch, S.V.; Matin, A.
    ChrR, a soluble quinone reductase of Pseudomonas putida that defends against H2O2. (2005), J. Biol. Chem., 280, 22590-22595.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information about 10fold induction of enzyme expression by H2O2 Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
gene chrR, quantitative expression analysis and profile Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information construction of ChrR overexpressing and knockout mutant strains showing increased and abolished tolerance of H2O2, respectively, enhancing the activity of ChrR in a chromate-remediating bacterial strain may not only increase the rate of chromate transformation, it may also augment the capacity of these cells to withstand the unavoidable production of H2O2 that accompanies chromate reduction, overview Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetic analysis of the quinone reductase activity Pseudomonas putida
0.11
-
benzoquinone pH 7.5, 70°C Pseudomonas putida
0.12
-
coenzyme Q1 pH 8.5, 60°C Pseudomonas putida
0.21
-
duroquinone pH 8.0, 70°C Pseudomonas putida
0.23
-
menadione pH 8.0, 60°C Pseudomonas putida
0.26
-
chromate(IV) pH 5.0, 70°C Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Pseudomonas putida
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida ChrR minimizes intracellular H2O2 stress ?
-
?
additional information Pseudomonas putida KT 2240 ChrR minimizes intracellular H2O2 stress ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
gene chrR
-
Pseudomonas putida KT 2240
-
gene chrR
-

Reaction

Reaction Comment Organism Reaction ID
NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone reaction mechanism Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
cell culture H2O2-scavenging and growth of wild-type, overexpressing, and knockout cells, overview Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoquinone + NADH
-
Pseudomonas putida ?
-
?
benzoquinone + NADH
-
Pseudomonas putida KT 2240 ?
-
?
chromate(IV) + H2O
-
Pseudomonas putida chromate(III) + H2O2
-
?
chromate(IV) + H2O
-
Pseudomonas putida KT 2240 chromate(III) + H2O2
-
?
coenzyme Q1 + NADH
-
Pseudomonas putida ?
-
?
duroquinone + NADH
-
Pseudomonas putida ?
-
?
menadione + NADH
-
Pseudomonas putida ?
-
?
menadione + NADH
-
Pseudomonas putida KT 2240 ?
-
?
additional information ChrR minimizes intracellular H2O2 stress Pseudomonas putida ?
-
?
additional information ChrR reduces quinones by simultaneous two-electron transfer, avoiding formation of highly reactive semiquinone intermediates and producing quinols that promote tolerance of H2O2 Pseudomonas putida ?
-
?
additional information ChrR minimizes intracellular H2O2 stress Pseudomonas putida KT 2240 ?
-
?
additional information ChrR reduces quinones by simultaneous two-electron transfer, avoiding formation of highly reactive semiquinone intermediates and producing quinols that promote tolerance of H2O2 Pseudomonas putida KT 2240 ?
-
?

Synonyms

Synonyms Comment Organism
chromate reductase
-
Pseudomonas putida
ChrR
-
Pseudomonas putida
Quinone reductase
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.8
-
chromate(IV) pH 5.0, 70°C Pseudomonas putida
6.4
-
coenzyme Q1 pH 8.5, 60°C Pseudomonas putida
25
-
duroquinone pH 8.0, 70°C Pseudomonas putida
440
-
menadione pH 8.0, 60°C Pseudomonas putida
930
-
benzoquinone pH 7.5, 70°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
chromate reductase activity Pseudomonas putida
7.5 8.5 quinone reductase activity Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
flavin flavoenzyme Pseudomonas putida
NADH
-
Pseudomonas putida