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Literature summary for 1.6.5.2 extracted from

  • Constam, D.; Muheim, A.; Zimmermann, W.; Fiechter, A.
    Purification and partial characterization of an intracellular NADH:quinone oxidoreductase from Phanerochaete chrysosporium (1991), J. Gen. Microbiol., 137, 2209-2214.
No PubMed abstract available
Search for more literature for 1.6.5.2

Inhibitors

Inhibitors Comment Organism Structure
3,3'-methylene-bis(4-hydroxycoumarin) trivial name dicoumarol, slight inhibition Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 NADH
-
 Phanerodontia chrysosporium
0.115
-
 2-methoxy-5-(methoxymethyl)-2,5-cyclohexadiene
-
 Phanerodontia chrysosporium
0.115
-
 2-methoxy-5-(methoxymethyl)-2,5-cyclohexadiene-1,4-dione
-
 Phanerodontia chrysosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Phanerodontia chrysosporium 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000 69000 gel filtration, SDS-PAGE Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Phanerodontia chrysosporium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxymethyl-5-methoxy-2,5-cyclohexadiene-1,4-dione + NAD(P)H
-
 Phanerodontia chrysosporium 2-hydroxymethyl-5-methoxy-2,5-cyclohexadiene-1,4-diol + NAD(P)+
-
 ?
2-methoxy-2,5-cyclohexadiene-1,4-dione + NAD(P)H
-
 Phanerodontia chrysosporium 2-methoxy-2,5-cyclohexadiene-1,4-diol + NAD(P)+
-
 ?
2-methoxy-5-(methoxymethyl)-2,5-cyclohexadiene + NADH + H+
-
 Phanerodontia chrysosporium 2-methoxy-5-methoxymethyl-2,5-cyclohexadiene-1,4-diol + NAD+
-
 r
2-methoxy-5-(methoxymethyl)-2,5-cyclohexadiene-1,4-dione + NAD(P)H
-
 Phanerodontia chrysosporium 2-methoxy-5-methoxymethyl-2,5-cyclohexadiene-1,4-diol + NAD(P)+
-
 ?
4,5-dimethoxy-3,5-cyclohexadiene-1,2-dione
-
 Phanerodontia chrysosporium 4,5-dimethoxy-3,5-cyclohexadiene-1,2-diol + NAD(P)+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 2-methoxy-5-(methoxymethyl)-2,5-cyclohexadiene Phanerodontia chrysosporium
8.5
-
 2-hydroxymethyl-5-methoxy-2,5-cyclohexadiene-1,4-dione Phanerodontia chrysosporium