Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Lactiplantibacillus pentosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0179 | - |
NADH | at pH 7.0 and 37°C | Lactiplantibacillus pentosus | |
1.3 | - |
BNAH | at pH 7.0 and 37°C | Lactiplantibacillus pentosus | |
1.6 | - |
MNAH | at pH 7.0 and 37°C | Lactiplantibacillus pentosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + O2 | Lactiplantibacillus pentosus | - |
NAD+ + H2O | - |
? | |
NADH + H+ + O2 | Lactiplantibacillus pentosus MP-10 | - |
NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus pentosus | F6IXY6 | - |
- |
Lactiplantibacillus pentosus MP-10 | F6IXY6 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and HiTrap Blue column chromatography | Lactiplantibacillus pentosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
BNAH + H+ + O2 | i.e. N-benzyl-1,4-dihydronicotinamide | Lactiplantibacillus pentosus | BNA+ + H2O | - |
? | |
BNAH + H+ + O2 | i.e. N-benzyl-1,4-dihydronicotinamide | Lactiplantibacillus pentosus MP-10 | BNA+ + H2O | - |
? | |
MNAH + H+ + O2 | i.e. N-methyl-1,4-dihydronicotinamide | Lactiplantibacillus pentosus | MNA+ + H2O | - |
? | |
MNAH + H+ + O2 | i.e. N-methyl-1,4-dihydronicotinamide | Lactiplantibacillus pentosus MP-10 | MNA+ + H2O | - |
? | |
additional information | NADPH can be recognized as a substrate by the enzyme, though the specific activity is only about 2% compared to NADH | Lactiplantibacillus pentosus | ? | - |
? | |
additional information | NADPH can be recognized as a substrate by the enzyme, though the specific activity is only about 2% compared to NADH | Lactiplantibacillus pentosus MP-10 | ? | - |
? | |
NADH + H+ + O2 | - |
Lactiplantibacillus pentosus | NAD+ + H2O | - |
? | |
NADH + H+ + O2 | - |
Lactiplantibacillus pentosus MP-10 | NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51940, calculated from amino acid sequence | Lactiplantibacillus pentosus |
Synonyms | Comment | Organism |
---|---|---|
NOX | - |
Lactiplantibacillus pentosus |
water-forming NADH oxidase | - |
Lactiplantibacillus pentosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Lactiplantibacillus pentosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
MNAH | at pH 7.0 and 37°C | Lactiplantibacillus pentosus | |
0.17 | - |
BNAH | at pH 7.0 and 37°C | Lactiplantibacillus pentosus | |
43.4 | - |
NADH | at pH 7.0 and 37°C | Lactiplantibacillus pentosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Lactiplantibacillus pentosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 70% activity at pH 6.0 and pH 8.0 | Lactiplantibacillus pentosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 13% FAD | Lactiplantibacillus pentosus | |
NADH | - |
Lactiplantibacillus pentosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
MNAH | at pH 7.0 and 37°C | Lactiplantibacillus pentosus | |
0.13 | - |
BNAH | at pH 7.0 and 37°C | Lactiplantibacillus pentosus |