Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | strong inhibition | Anaerocolumna aminovalerica | |
p-chloromercuribenzoate | strong inhibition | Anaerocolumna aminovalerica | |
quinacrin | strong inhibition | Anaerocolumna aminovalerica | |
Quinine | strong inhibition | Anaerocolumna aminovalerica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0192 | - |
NADH | pH 7.0, 25°C | Anaerocolumna aminovalerica | |
0.0619 | - |
O2 | pH 7.0, 25°C | Anaerocolumna aminovalerica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Anaerocolumna aminovalerica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 NADH + H+ + O2 | Anaerocolumna aminovalerica | the enzyme is involved detoxification of oxygen to water | NAD+ + 2 H2O | - |
? | |
2 NADH + H+ + O2 | Anaerocolumna aminovalerica NRIC0223 | the enzyme is involved detoxification of oxygen to water | NAD+ + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anaerocolumna aminovalerica | Q2WFW5 | - |
- |
Anaerocolumna aminovalerica NRIC0223 | Q2WFW5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Anaerocolumna aminovalerica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130 | - |
pH 7.0, 37°C | Anaerocolumna aminovalerica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 NADH + H+ + O2 | the enzyme is involved detoxification of oxygen to water | Anaerocolumna aminovalerica | NAD+ + 2 H2O | - |
? | |
2 NADH + H+ + O2 | the enzyme can catalyze electron transfers from NADH to ferricyanide, 2,6-dichloroindophenol, and menadione, although the activities with these acceptors are extremely low relative to O2. Thus, O2 must be a natural electron acceptor for this enzyme | Anaerocolumna aminovalerica | NAD+ + 2 H2O | - |
? | |
2 NADH + H+ + O2 | the enzyme is involved detoxification of oxygen to water | Anaerocolumna aminovalerica NRIC0223 | NAD+ + 2 H2O | - |
? | |
2 NADH + H+ + O2 | the enzyme can catalyze electron transfers from NADH to ferricyanide, 2,6-dichloroindophenol, and menadione, although the activities with these acceptors are extremely low relative to O2. Thus, O2 must be a natural electron acceptor for this enzyme | Anaerocolumna aminovalerica NRIC0223 | NAD+ + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 4500, SDS-PAGE | Anaerocolumna aminovalerica |
Synonyms | Comment | Organism |
---|---|---|
H2O-forming NADH oxidase | - |
Anaerocolumna aminovalerica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
20 min, stable | Anaerocolumna aminovalerica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Anaerocolumna aminovalerica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, contains 34 mol FAD per mol native enzyme. The NADH oxidase activity is not stimulated by the addition of free flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), or riboflavin to the reaction mixture (final concentrations were 0.1 mM) | Anaerocolumna aminovalerica |