Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 250 mM, 10fold activation | Brevibacterium sp. | |
p-chloromercuribenzoate | 1 mM, 1.9fold activation | Brevibacterium sp. |
Application | Comment | Organism |
---|---|---|
synthesis | regeneration of NAD+ utilizing this enzyme made selective oxidation of mandelic acid or L-phenylalanine possible. This thermostable enzyme is expected to be applicable as a useful biocatalyst for NAD+ recycling | Brevibacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM, 63% inhibition | Brevibacterium sp. | |
CuCl2 | 1 mM, 58% inhibition | Brevibacterium sp. | |
HgCl2 | 1 mM, 29% inhibition | Brevibacterium sp. | |
ZnCl2 | 1 mM, 61% inhibition | Brevibacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
NADH | pH 8.8, 25°C | Brevibacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Brevibacterium sp. | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, SDS-PAGE | Brevibacterium sp. |
102000 | - |
gel filtration | Brevibacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + O2 | Brevibacterium sp. | the enzyme is highly specific for NADH, no activity with NADPH | NAD+ + H2O2 | - |
? | |
NADH + H+ + O2 | Brevibacterium sp. KU1309 | the enzyme is highly specific for NADH, no activity with NADPH | NAD+ + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sp. | - |
- |
- |
Brevibacterium sp. KU1309 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevibacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:2-phenylethanol-grown cell | - |
Brevibacterium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.2 | - |
pH 8.8, 25°C | Brevibacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + O2 | the enzyme is highly specific for NADH, no activity with NADPH | Brevibacterium sp. | NAD+ + H2O2 | - |
? | |
NADH + H+ + O2 | the enzyme is highly specific for NADH, no activity with NADPH | Brevibacterium sp. KU1309 | NAD+ + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, SDS-PAGE | Brevibacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
H2O2-forming NADH oxidase | - |
Brevibacterium sp. |
NOX | - |
Brevibacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Brevibacterium sp. |
70 | - |
- |
Brevibacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | activity at 30°C is about 40% of the maximal at 70°C | Brevibacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1 h, no significant loss of activity | Brevibacterium sp. |
80 | - |
1 h, about 30% loss of activity | Brevibacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Brevibacterium sp. |
9 | - |
- |
Brevibacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.9 | 11 | pH 7.8: about 60% of maximal activity, pH 11.0: about 90% of maximal activity | Brevibacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 1 h, stable | Brevibacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzyme is highly specific for NADH, relative activity with NADPH is 2% compared to the activity with NADH | Brevibacterium sp. |