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Literature summary for 1.6.3.1 extracted from

  • Arcari, P.; Masullo, L.; Masullo, M.; Catanzano, F.; Bocchini, V.
    A NAD(P)H oxidase isolated from the archaeon Sulfolobus solfataricus is not homologous with another NADH oxidase present in the same microorganism. Biochemical characterization of the enzyme and cloning of the encoding gene (2000), J. Biol. Chem., 275, 895-900.
    View publication on PubMed
Search for more literature for 1.6.3.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharolobus solfataricus

General Stability

General Stability Organism
presence of trypsin at a 3:1 weight ratio Saccharolobus solfataricus
resistance to trypsin, cleavage of in two major fragments of about 21 and 14 kDa is obtained after incubation of the protein for 20 h at 37°C in the Saccharolobus solfataricus
the enzyme is resistant against chemical and physical denaturating agents Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.413
-
 NADH pH 5.5, 65°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36508
-
 2 * 36508, calculated from sequence Saccharolobus solfataricus
38000
-
 2 * 38000, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 pH 5.5, 65°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + O2 oxidizes NADH and, less efficiently, NADPH Saccharolobus solfataricus NAD+ + H2O2
-
 ?
NADH + H+ + O2 oxidizes NADH and, less efficiently, NADPH Saccharolobus solfataricus MT-4 / DSM 5833 NAD+ + H2O2
-
 ?
NADPH + H+ + O2 oxidizes NADH and, less efficiently, NADPH (2fold lower activity) Saccharolobus solfataricus NADP+ + H2O2
-
 ?
NADPH + H+ + O2 oxidizes NADH and, less efficiently, NADPH (2fold lower activity) Saccharolobus solfataricus MT-4 / DSM 5833 NADP+ + H2O2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Saccharolobus solfataricus
homodimer 2 * 36508, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SsNOX38
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Saccharolobus solfataricus
87
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 95 80°C: about 45% of maximal activity, 95°C: about 45% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 temperature for half-denaturation in presence of 8 M urea Saccharolobus solfataricus
87
-
 the enzyme remains stable for 60 min Saccharolobus solfataricus
93
-
 temperature for half-denaturation in absence of 8 M urea Saccharolobus solfataricus
105
-
 t1/2: about 2 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 NADH pH 5.5, 65°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
FAD contains 1 FAD molecule/subunit, the enzyme requires the presence of FAD as cofactor Saccharolobus solfataricus
NADH oxidizes NADH and, less efficiently, NADPH Saccharolobus solfataricus
NADPH 2fold lower activity compared to NADH Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.7
-
 NADH pH 5.5, 65°C Saccharolobus solfataricus