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Literature summary for 1.6.2.6 extracted from

  • Luan, P.; Arechaga-Ocampo, E.; Sarath, G.; Arredondo-Peter, R.; Klucas, R.V.
    Analysis of a ferric leghemoglobin reductase from cowpea (Vigna unguiculata) root nodules (2000), Plant Sci., 154, 161-170.
    View publication on PubMed
Search for more literature for 1.6.2.6

Cloned(Commentary)

Cloned (Comment) Organism
cloning of cDNA Vigna unguiculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0104
-
 ferrileghemoglobin cowpea ferrileghemoglobin Vigna unguiculata
0.0124
-
 ferrileghemoglobin soybean ferrileghemoglobin Vigna unguiculata
0.057
-
 NADH
-
 Vigna unguiculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 2 * 55000, SDS-PAGE Vigna unguiculata
56000
-
 2 * 56000, deduced from amino acid sequence Vigna unguiculata
110000
-
 gel filtration Vigna unguiculata

Organism

Organism UniProt Comment Textmining
Vigna unguiculata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, hydroxylapatite, ion exchange, gel filtration Vigna unguiculata

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
 Vigna unguiculata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.216
-
 reduction of ferrileghemoglobin Vigna unguiculata
2.565
-
 reduction of 2,6-dichloroindophenol Vigna unguiculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dichloroindophenol + NADH
-
 Vigna unguiculata reduced 2,6-dichloroindophenol + NAD+
-
 ?
NAD(P)H + ferrileghemoglobin reduces both cowpea ferrihemoglobin and soybean ferrileghemoglobin Vigna unguiculata NAD(P)+ + ferroleghemoglobin
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE Vigna unguiculata
dimer 2 * 56000, deduced from amino acid sequence Vigna unguiculata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 ferrileghemoglobin soybean ferrileghemoglobin Vigna unguiculata
3.1
-
 ferrileghemoglobin cowpea ferrileghemoglobin Vigna unguiculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 reduction of ferrileghemoglobin Vigna unguiculata

pH Range

pH Minimum pH Maximum Comment Organism
4.8 8.5 no activity below and above Vigna unguiculata