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Literature summary for 1.6.2.6 extracted from
- Molecular cloning, functional characterization, and subcellular localization of soybean nodule dihydrolipoamide reductase (2002), Plant Physiol., 128, 300-313.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21 | Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
ferrileghemoglobin | recombinant FLbR-2 | Glycine max |  |
| 0.058 | - |
NADH | recombinant FLbR-2 | Glycine max | |
| 3.381 | - |
Lipoamide | recombinant FLbR-2 | Glycine max | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Glycine max | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50030 | - |
electrospray mass spectrometry | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | O81413 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Glycine max | - |
| root | - |
Glycine max | - |
| root nodule | - |
Glycine max | - |
| stem | - |
Glycine max | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + ferrileghemoglobin | - |
Glycine max | NAD+ + ferroleghemoglobin | - |
? | |
| NADH + lipoamide | - |
Glycine max | NAD+ + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FLbR-2 | - |
Glycine max |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
ferrileghemoglobin | recombinant FLbR-2 | Glycine max | |
| 137 | - |
NADH | recombinant FLbR-2 | Glycine max | |
| 392 | - |
Lipoamide | recombinant FLbR-2 | Glycine max | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | not covalently bound to the protein | Glycine max | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Glycine max | isoelectric focusing, pH-range 4.0-6.5 | - |
5.8 |
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