BRENDA - Enzyme Database
show all sequences of 1.6.2.5

Isolation, purification, and some properties of reduced nicotinamide adenine dinucleotide phosphate-cytochrome C2 reductase from Rhodopseudomonas spheroides

Sabo, D.J.; Orlando, J.A.; J. Biol. Chem. 243, 3742-3749 (1968)

Data extracted from this reference:

General Stability
General Stability
Organism
activity is lost overnight if the enzyme is diluted
Luteovulum sphaeroides
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetate
-
Luteovulum sphaeroides
N-ethylmaleimide
-
Luteovulum sphaeroides
NADH
competitive inhibitor
Luteovulum sphaeroides
p-chloromercuribenzoate
-
Luteovulum sphaeroides
thyroxine
-
Luteovulum sphaeroides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0125
-
2,6-dichloroindophenol
-
Luteovulum sphaeroides
0.037
-
ferricytochrome c2
-
Luteovulum sphaeroides
0.125
-
ferricytochrome c2
-
Luteovulum sphaeroides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
Luteovulum sphaeroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2 ferricytochrome c2 + NADPH
Rhodopseudomonas palustris
-
2 ferrocytochrome c2 + NADP+ + H+
-
-
?
2 ferricytochrome c2 + NADPH
Luteovulum sphaeroides
native cytochrome is the natural physiological electron acceptor
2 ferrocytochrome c2 + NADP+ + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Luteovulum sphaeroides
-
-
-
Rhodopseudomonas palustris
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Luteovulum sphaeroides
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
164
-
-
Luteovulum sphaeroides
Storage Stability
Storage Stability
Organism
-15C, can be stored as lyophilized powder for at least 3 months
Luteovulum sphaeroides
4C, 0.4 M Tris-HCl, pH 8.0 purified concentrated enzyme is stable for approximately 2 weeks, gradual loss in activity can be restored by addition of glutathione to a final concentration of 0.0001 M, partially purified enzyme is stable for approximately 1 month
Luteovulum sphaeroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2 ferricytochrome c2 + NADPH
-
437739
Rhodopseudomonas palustris
2 ferrocytochrome c2 + NADP+ + H+
-
-
-
?
2 ferricytochrome c2 + NADPH
contains additional NADP+ diaphorase activity
437739
Luteovulum sphaeroides
2 ferrocytochrome c2 + NADP+ + H+
-
-
-
?
2 ferricytochrome c2 + NADPH
contains additional NADP+ diaphorase activity
437739
Rhodopseudomonas palustris
2 ferrocytochrome c2 + NADP+ + H+
-
-
-
?
2 ferricytochrome c2 + NADPH
native cytochrome is the natural physiological electron acceptor
437739
Luteovulum sphaeroides
2 ferrocytochrome c2 + NADP+ + H+
-
-
-
?
2,6-dichloroindophenol + NADPH
-
437739
Luteovulum sphaeroides
? + NADP+
-
-
-
?
Fe(CN)6 3- + NADPH
-
437739
Luteovulum sphaeroides
Fe(CN)6 4- + NADP+
-
-
-
?
horse heart cytochrome c + NADPH
-
437739
Luteovulum sphaeroides
? + NADP+
-
-
-
?
mammalian cytochrome c + NADPH
-
437739
Luteovulum sphaeroides
? + NADP+
-
-
-
?
additional information
cytochrome c-553 is ineffective as an electron acceptor
437739
Luteovulum sphaeroides
?
-
-
-
?
NADPH + H+ + ferricytochrome c2
-
437739
Luteovulum sphaeroides
NADP+ + ferrocytochrome c2
-
-
-
r
Rhodospirillum rubrum cytochrome c2 + NADPH
-
437739
Luteovulum sphaeroides
? + NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
NADPH-cytochrome f reductase
-
Luteovulum sphaeroides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Luteovulum sphaeroides
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.6
8
-
Luteovulum sphaeroides
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
nonmetalloflavoprotein with FAD as prosthetic group
Luteovulum sphaeroides
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.055
-
NADH
-
Luteovulum sphaeroides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
nonmetalloflavoprotein with FAD as prosthetic group
Luteovulum sphaeroides
General Stability (protein specific)
General Stability
Organism
activity is lost overnight if the enzyme is diluted
Luteovulum sphaeroides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetate
-
Luteovulum sphaeroides
N-ethylmaleimide
-
Luteovulum sphaeroides
NADH
competitive inhibitor
Luteovulum sphaeroides
p-chloromercuribenzoate
-
Luteovulum sphaeroides
thyroxine
-
Luteovulum sphaeroides
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.055
-
NADH
-
Luteovulum sphaeroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0125
-
2,6-dichloroindophenol
-
Luteovulum sphaeroides
0.037
-
ferricytochrome c2
-
Luteovulum sphaeroides
0.125
-
ferricytochrome c2
-
Luteovulum sphaeroides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
Luteovulum sphaeroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2 ferricytochrome c2 + NADPH
Rhodopseudomonas palustris
-
2 ferrocytochrome c2 + NADP+ + H+
-
-
?
2 ferricytochrome c2 + NADPH
Luteovulum sphaeroides
native cytochrome is the natural physiological electron acceptor
2 ferrocytochrome c2 + NADP+ + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Luteovulum sphaeroides
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
164
-
-
Luteovulum sphaeroides
Storage Stability (protein specific)
Storage Stability
Organism
-15C, can be stored as lyophilized powder for at least 3 months
Luteovulum sphaeroides
4C, 0.4 M Tris-HCl, pH 8.0 purified concentrated enzyme is stable for approximately 2 weeks, gradual loss in activity can be restored by addition of glutathione to a final concentration of 0.0001 M, partially purified enzyme is stable for approximately 1 month
Luteovulum sphaeroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2 ferricytochrome c2 + NADPH
-
437739
Rhodopseudomonas palustris
2 ferrocytochrome c2 + NADP+ + H+
-
-
-
?
2 ferricytochrome c2 + NADPH
contains additional NADP+ diaphorase activity
437739
Luteovulum sphaeroides
2 ferrocytochrome c2 + NADP+ + H+
-
-
-
?
2 ferricytochrome c2 + NADPH
contains additional NADP+ diaphorase activity
437739
Rhodopseudomonas palustris
2 ferrocytochrome c2 + NADP+ + H+
-
-
-
?
2 ferricytochrome c2 + NADPH
native cytochrome is the natural physiological electron acceptor
437739
Luteovulum sphaeroides
2 ferrocytochrome c2 + NADP+ + H+
-
-
-
?
2,6-dichloroindophenol + NADPH
-
437739
Luteovulum sphaeroides
? + NADP+
-
-
-
?
Fe(CN)6 3- + NADPH
-
437739
Luteovulum sphaeroides
Fe(CN)6 4- + NADP+
-
-
-
?
horse heart cytochrome c + NADPH
-
437739
Luteovulum sphaeroides
? + NADP+
-
-
-
?
mammalian cytochrome c + NADPH
-
437739
Luteovulum sphaeroides
? + NADP+
-
-
-
?
additional information
cytochrome c-553 is ineffective as an electron acceptor
437739
Luteovulum sphaeroides
?
-
-
-
?
NADPH + H+ + ferricytochrome c2
-
437739
Luteovulum sphaeroides
NADP+ + ferrocytochrome c2
-
-
-
r
Rhodospirillum rubrum cytochrome c2 + NADPH
-
437739
Luteovulum sphaeroides
? + NADP+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Luteovulum sphaeroides
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.6
8
-
Luteovulum sphaeroides
Other publictions for EC 1.6.2.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
437741
Norrheim
Synergistic actions of tetrade ...
Homo sapiens, Rattus norvegicus, Rattus norvegicus Wistar
Biochim. Biophys. Acta
1051
319-323
1990
-
-
-
-
-
-
-
-
-
-
-
3
-
169
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437740
Feuers
Assay for mouse tissue enzymes ...
Mus musculus
Anal. Biochem.
101
123-130
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437739
Sabo
Isolation, purification, and s ...
Luteovulum sphaeroides, Rhodopseudomonas palustris
J. Biol. Chem.
243
3742-3749
1968
-
-
-
-
-
1
5
3
-
-
1
2
-
2
-
-
1
-
-
-
1
2
11
-
1
-
-
-
-
1
1
-
1
1
-
-
-
-
-
1
-
-
1
-
5
1
3
-
-
1
2
-
-
-
1
-
-
1
2
11
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-