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Literature summary for 1.6.2.4 extracted from

  • Etzerodt, T.; Wetterhorn, K.; Dionisio, G.; Rayment, I.
    Functional characterization of a soluble NADPH-cytochrome P450 reductase from Fusarium graminearum (2017), Protein Expr. Purif., 138, 69-75 .
    View publication on PubMed
Search for more literature for 1.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli codon plus cells Fusarium graminearum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0189
-
 ferricytochrome c at pH 8.5 and 23°C Fusarium graminearum
0.0439
-
 ferricytochrome c at pH 7.5 and 23°C Fusarium graminearum
0.0746
-
 ferricytochrome c at pH 9.5 and 23°C Fusarium graminearum

Organism

Organism UniProt Comment Textmining
Fusarium graminearum
-
-
-
Fusarium graminearum PH-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Fusarium graminearum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + ferricytochrome c
-
 Fusarium graminearum NADP+ + ferrocytochrome c
-
 ?
NADPH + H+ + ferricytochrome c
-
 Fusarium graminearum PH-1 NADP+ + ferrocytochrome c
-
 ?
NADPH + H+ + oxidized thiazolyl blue tetrazolium bromide
-
 Fusarium graminearum NADP+ + reduced thiazolyl blue tetrazolium bromide
-
 ?
NADPH + H+ + oxidized thiazolyl blue tetrazolium bromide
-
 Fusarium graminearum PH-1 NADP+ + reduced thiazolyl blue tetrazolium bromide
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Fusarium graminearum

Synonyms

Synonyms Comment Organism
CPR
-
 Fusarium graminearum
NADPH-cytochrome P450 reductase
-
 Fusarium graminearum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Fusarium graminearum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Fusarium graminearum
FMN
-
 Fusarium graminearum