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Literature summary for 1.6.2.4 extracted from

  • Qu, X.; Pu, X.; Chen, F.; Yang, Y.; Yang, L.; Zhang, G.; Luo, Y.
    Molecular cloning, heterologous expression, and functional characterization of an NADPH-cytochrome P450 reductase gene from Camptotheca acuminata, a camptothecin-producing plant (2015), PLoS ONE, 10, e0135397 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Camptotheca acuminata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0187
-
 NADPH at pH 7.4 and 25°C Camptotheca acuminata
0.0514
-
 ferricytochrome c at pH 7.4 and 25°C Camptotheca acuminata
0.1193
-
 ferricyanide at pH 7.4 and 25°C Camptotheca acuminata

Organism

Organism UniProt Comment Textmining
Camptotheca acuminata A0A0D5BVX3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography, and Superdex 200 gel filtration Camptotheca acuminata

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Camptotheca acuminata
-
root
-
 Camptotheca acuminata
-
stem
-
 Camptotheca acuminata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with NADH Camptotheca acuminata ?
-
 ?
NADPH + H+ + ferricyanide
-
 Camptotheca acuminata NADP+ + ferrocyanide
-
 ?
NADPH + H+ + ferricytochrome c
-
 Camptotheca acuminata NADP+ + ferrocytochrome c
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Camptotheca acuminata

Synonyms

Synonyms Comment Organism
CPR
-
 Camptotheca acuminata
NADPH-cytochrome P450 reductase
-
 Camptotheca acuminata

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Camptotheca acuminata
FMN
-
 Camptotheca acuminata