Literature summary for 1.6.2.4 extracted from

Jang, H.H.; Jamakhandi, A.P.; Sullivan, S.Z.; Yun, C.H.; Hollenberg, P.F.; Miller, G.P.
Beta sheet 2-alpha helix C loop of cytochrome P450 reductase serves as a docking site for redox partners (2010), Biochim. Biophys. Acta, 1804, 1285-1293.

Protein Variants

Protein Variants	Comment	Organism
D113A	the mutation increases kcat approximately 2fold, but does not affect Km at the lowest ionic strength (10 mM), the mutant displays no change in catalytic efficiency compared to the wild type enzyme	Rattus norvegicus
E115A/E116A	the mutations increase kcat approximately 2fold, but does not affect Km at the lowest ionic strength (10 mM), the mutant displays a slight decrease in catalytic efficiency at higher ionic strengths due to a larger increase in Km than observed for kcat	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0018	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.002	-	ferricytochrome c	wild type enzyme, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0021	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0022	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0024	-	ferricytochrome c	mutant enzyme E115A/E116A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0025	-	ferricytochrome c	mutant enzyme D113A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0026	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0026	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0031	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0036	-	ferricytochrome c	mutant enzyme E115A/E116A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0038	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0039	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.004	-	ferricytochrome c	wild type enzyme, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0041	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0052	-	ferricytochrome c	mutant enzyme D113A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.006	-	ferricytochrome c	mutant enzyme E115A/E116A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0062	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0063	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0065	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0085	-	ferricytochrome c	wild type enzyme, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.0087	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.01	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.011	-	ferricytochrome c	mutant enzyme D113A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.011	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.013	-	ferricytochrome c	mutant enzyme E115A/E116A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.016	-	ferricytochrome c	mutant enzyme E115A/E116A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.02	-	ferricytochrome c	wild type enzyme, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.023	-	ferricytochrome c	wild type enzyme, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.025	-	ferricytochrome c	mutant enzyme D113A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
0.026	-	ferricytochrome c	mutant enzyme D113A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
78000	-	SDS-PAGE	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 ferricytochrome P450 + NADPH	Rattus norvegicus	-	2 ferrocytochrome P450 + NADP+ + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
2',5'-ADP agarose affinity column chromatography and DEAE resin column chromatography	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2 ferricytochrome c + NADPH	-	Rattus norvegicus	2 ferrocytochrome c + NADP+ + H+	-	?
2 ferricytochrome P450 + NADPH	-	Rattus norvegicus	2 ferrocytochrome P450 + NADP+ + H+	-	?
NADPH + H+ + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	-	Rattus norvegicus	NADP+ + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	-	?

Synonyms

Synonyms	Comment	Organism
CPR	-	Rattus norvegicus
cytochrome P450 reductase	-	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
5.83	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
8.5	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
10.5	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
11.67	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
13	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
13.83	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
14.67	-	ferricytochrome c	wild type enzyme, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
16.67	-	ferricytochrome c	wild type enzyme, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
18.3	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
18.3	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
20	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
20	-	ferricytochrome c	wild type enzyme, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
23.3	-	ferricytochrome c	mutant enzyme D113A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
25	-	ferricytochrome c	mutant enzyme D113A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
25	-	ferricytochrome c	mutant enzyme E115A/E116A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
25	-	ferricytochrome c	mutant enzyme E115A/E116A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
25	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
28.3	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
30	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
33.33	-	ferricytochrome c	wild type enzyme, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
35	-	ferricytochrome c	wild type enzyme, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
36.7	-	ferricytochrome c	mutant enzyme D113A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
36.7	-	ferricytochrome c	mutant enzyme E115A/E116A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
38.3	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
40	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
55	-	ferricytochrome c	mutant enzyme D113A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
56.67	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
58.3	-	ferricytochrome c	mutant enzyme E115A/E116A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
58.3	-	ferricytochrome c	mutant enzyme E115A/E116A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
65	-	ferricytochrome c	mutant enzyme D113A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism
FAD	-	Rattus norvegicus
FMN	-	Rattus norvegicus
NADPH	-	Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
1500	-	ferricytochrome c	wild type enzyme, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
1700	-	ferricytochrome c	wild type enzyme, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
2200	-	ferricytochrome c	mutant enzyme D113A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
2300	-	ferricytochrome c	wild type enzyme, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
2500	-	ferricytochrome c	mutant enzyme D113A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
2700	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
2700	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
2800	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
3000	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
3000	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	wild type enzyme, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
3300	-	ferricytochrome c	mutant enzyme D113A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
3700	-	ferricytochrome c	mutant enzyme E115A/E116A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
3700	-	ferricytochrome c	wild type enzyme, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
4500	-	ferricytochrome c	mutant enzyme D113A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
4500	-	ferricytochrome c	mutant enzyme E115A/E116A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
4700	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
4800	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
4800	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
5000	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
5000	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
5300	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme D113A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
5800	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
6000	-	ferricytochrome c	mutant enzyme E115A/E116A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
6500	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
6800	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
7000	-	ferricytochrome c	mutant enzyme E115A/E116A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
7200	-	oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide	mutant enzyme E115A/E116A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
8300	-	ferricytochrome c	wild type enzyme, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
10000	-	ferricytochrome c	mutant enzyme D113A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus
10500	-	ferricytochrome c	mutant enzyme E115A/E116A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C	Rattus norvegicus