Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL-1 Blue cells | Anopheles minimus |
Protein Variants | Comment | Organism |
---|---|---|
L219F | the mutant upon reconstitution with the Anopheles minimus cytochrome P450 CYP6AA3 and a NADPH-regenerating system, increases CYP6AA3-mediated deltamethrin degradation compared to the wild type enzyme | Anopheles minimus |
L86F | the mutant upon reconstitution with the Anopheles minimus cytochrome P450 CYP6AA3 and a NADPH-regenerating system, increases CYP6AA3-mediated deltamethrin degradation compared to the wild type enzyme | Anopheles minimus |
L86F/L219F | the mutant upon reconstitution with the Anopheles minimus cytochrome P450 CYP6AA3 and a NADPH-regenerating system, increases CYP6AA3-mediated deltamethrin degradation compared to the wild type enzyme | Anopheles minimus |
additional information | DELTA55AnCYPOR has an approximately 0.5fold increased FAD content compared to the wild type enzyme | Anopheles minimus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | competitive inhibition | Anopheles minimus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0116 | - |
NADPH | mutant enzyme L86F/L219F, pH not specified in the publication, at 25°C | Anopheles minimus | |
0.0125 | - |
NADPH | wild type enzyme, pH not specified in the publication, at 25°C | Anopheles minimus | |
0.019 | - |
ferricytochrome c | wild type enzyme, pH not specified in the publication, at 25°C | Anopheles minimus | |
0.0239 | - |
ferricytochrome c | mutant enzyme L86F/L219F, pH not specified in the publication, at 25°C | Anopheles minimus | |
4 | - |
NADH | wild type enzyme, pH not specified in the publication, at 25°C | Anopheles minimus | |
4.3 | - |
NADH | mutant enzyme L86F/L219F, pH not specified in the publication, at 25°C | Anopheles minimus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Anopheles minimus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
His-tagged enzyme, SDS-PAGE | Anopheles minimus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome P450 + NADPH | Anopheles minimus | - |
2 ferrocytochrome P450 + NADP+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles minimus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex G25 gel filtration, the purified enzyme readily loses its flavin cofactors | Anopheles minimus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADH | lower binding affinity for NADH compared to NADPH | Anopheles minimus | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | - |
Anopheles minimus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome P450 + NADPH | - |
Anopheles minimus | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYPOR | - |
Anopheles minimus |
NADPH-cytochrome P450 oxidoreductase | - |
Anopheles minimus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains 0.22 mol of FAD per mol of protein, when supplemented with exogenous flavin cofactors, the activity of purified CYPOR-mediated cytochrome c reduction is increased | Anopheles minimus | |
FMN | the enzyme contains 0.11 mol of FMN per mol of protein, when supplemented with exogenous flavin cofactors, the activity of purified CYPOR-mediated cytochrome c reduction is increased | Anopheles minimus | |
NADPH | - |
Anopheles minimus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0115 | - |
NADP+ | wild type enzyme, pH not specified in the publication, at 25°C | Anopheles minimus |