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Literature summary for 1.6.2.4 extracted from

  • Bonina, T.A.; Gilep, A.A.; Estabrook, R.W.; Usanov, S.A.
    Engineering of proteolytically stable NADPH-cytochrome P450 reductase (2005), Biochemistry, 70, 357-365.
    View publication on PubMed
Search for more literature for 1.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant K56Q, expression in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
K56Q the full-length mutant enzyme is stable to spontaneopus proteolysis but possesses spectral and catzalytic properties of the wild-type flavoprotein Rattus norvegicus
additional information The removal of the N-terminal hydrophobic sequence of NADPH-cytochrome P450 reductase results in loss of the ability of the flavoprotein to interact and transfer electrons to cytochrome P450. Truncated forms of the flavoprotein (residue 46-676 of the mutant (Q56Q) or 57-676 of the wild-type NADPH-cytochrome P450 reductase) are unable to transfer electrons to cytochrome P450c17 or P4503A4 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ferricytochrome c + NADPH Rattus norvegicus NADPH-cytochrome P450 reductase is a unique universal donor of electrons to practically all known microsomal cytochrome P450s and one of the most important components of the monooxygenase system 2 ferrocytochrome c + NADP+ + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH
-
 Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
2 ferricytochrome c + NADPH NADPH-cytochrome P450 reductase is a unique universal donor of electrons to practically all known microsomal cytochrome P450s and one of the most important components of the monooxygenase system Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Rattus norvegicus