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Literature summary for 1.6.2.4 extracted from
- Catalytic and immunochemical properties of NADPH-cytochrome P450 reductase from fungus Rhizopus nigricans (2002), J. Steroid Biochem. Mol. Biol., 82, 89-96.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of trypsin significantly increases activity in detergent free buffer, no effect in the presence of detergent | Rhizopus stolonifer |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rhizopus stolonifer |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rhizopus stolonifer | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
SDS-PAGE, band is accompanied by two smaller, proteolytic forms of the enzyme formed during solubilization or purification | Rhizopus stolonifer |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome P450 + NADPH | Rhizopus stolonifer | involved in the hydroxylation of progesterone at the 11alpha position | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus stolonifer | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizopus stolonifer |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| NADPH + oxidized hemoprotein = NADP+ + reduced hemoprotein + H+ | at high ionic strength nonclassical two-site ping pong mechanism, at low ionic strength a sequential bisubstrate mechanism | Rhizopus stolonifer |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Rhizopus stolonifer | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | at high ionic strength nonclassical two-site ping pong mechanism, at low ionic strength a sequential bisubstrate mechanism | Rhizopus stolonifer | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome P450 + NADPH | involved in the hydroxylation of progesterone at the 11alpha position | Rhizopus stolonifer | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH-cytochrome P450 reductase | - |
Rhizopus stolonifer |
| NADPH-ferrihemoprotein reductase | - |
Rhizopus stolonifer |
| NADPH:ferrihemoprotein oxidoreductase | - |
Rhizopus stolonifer |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | together with FMN | Rhizopus stolonifer | |
| FMN | together with FAD | Rhizopus stolonifer | |
| NADPH | - |
Rhizopus stolonifer | |
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