Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
Y140D | substitution does not eliminate FMN binding but reduces cytochrome c reductase activity, Km value for cytochrome c or NADPH similar to wild type | Rattus norvegicus |
Y140D/178D | substitution abolishes FMN binding and cytochrome c reductase activity | Rattus norvegicus |
Y140F | substitution has no effect on FMN content or catalytic activity, Km value for cytochrome c or NADPH similar to wild type | Rattus norvegicus |
Y140F/178F | substitution has no effect on FMN content or catalytic activity, slightly decreases Km for cytochrome c, NADPH Km value slightly higher than wild type | Rattus norvegicus |
Y178D | substitution abolishes FMN binding and cytochrome c reductase activity, Km value for cytochrome c similar to wild type, NADPH Km value slightly higher than wild type | Rattus norvegicus |
Y178F | substitution has no effect on FMN content or catalytic activity, slightly decreases Km for cytochrome c, NADPH Km value similar to wild type | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
NADPH | wild type, cosubstrate cytochrome c | Rattus norvegicus | |
0.0066 | - |
NADPH | native enzyme, liver, cosubstrate cytochrome c | Rattus norvegicus | |
0.0066 | - |
NADPH | Y140D mutant, cosubstrate cytochrome c | Rattus norvegicus | |
0.0071 | - |
NADPH | Y178F mutant, cosubstrate cytochrome c | Rattus norvegicus | |
0.0078 | - |
NADPH | Y140F mutant, cosubstrate cytochrome c | Rattus norvegicus | |
0.0095 | - |
NADPH | Y178D mutant, cosubstrate cytochrome c | Rattus norvegicus | |
0.0114 | - |
NADPH | Y140F/178F double mutant, cosubstrate cytochrome c | Rattus norvegicus | |
0.0131 | - |
cytochrome c | Y140F/178F double mutant | Rattus norvegicus | |
0.014 | - |
cytochrome c | Y178F mutant | Rattus norvegicus | |
0.0178 | - |
cytochrome c | native enzyme, liver | Rattus norvegicus | |
0.0185 | - |
cytochrome c | Y178D mutant | Rattus norvegicus | |
0.0188 | - |
cytochrome c | Y140F mutant | Rattus norvegicus | |
0.0191 | - |
cytochrome c | Y140D mutant | Rattus norvegicus | |
0.0211 | - |
cytochrome c | wild type | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
wild type, x * 80000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
expressed in Escherichia coli | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
Y140D/178D double mutant, electron acceptor: cytochrome c | Rattus norvegicus |
0.44 | - |
Y178D mutant, electron acceptor: cytochrome c | Rattus norvegicus |
11 | - |
Y140D mutant, electron acceptor: cytochrome c | Rattus norvegicus |
46 | - |
Y140F/178F double mutant, electron acceptor: cytochrome c | Rattus norvegicus |
47.4 | - |
Y178F mutant, electron acceptor: cytochrome c | Rattus norvegicus |
48.1 | - |
Y178D mutant, electron acceptor: ferricyanide | Rattus norvegicus |
51 | - |
Y140D/178D double mutant, electron acceptor: ferricyanide | Rattus norvegicus |
51.5 | - |
bacterially expressed reductase protein, electron acceptor: cytochrome c | Rattus norvegicus |
52.9 | - |
electron acceptor: ferricyanide | Rattus norvegicus |
53.3 | - |
electron acceptor: cytochrome c | Rattus norvegicus |
55.2 | - |
Y140F mutant, electron acceptor: cytochrome c | Rattus norvegicus |
94.2 | - |
Y140F/178F double mutant, electron acceptor: ferricyanide | Rattus norvegicus |
94.8 | - |
Y140F mutant, electron acceptor: ferricyanide | Rattus norvegicus |
102 | - |
bacterially expressed reductase protein, electron acceptor: ferricyanide | Rattus norvegicus |
102 | - |
Y140D mutant, electron acceptor: ferricyanide | Rattus norvegicus |
113 | - |
Y178F mutant, electron acceptor: ferricyanide | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Rattus norvegicus | 2 ferrocytochrome c + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | wild type, x * 80000, SDS-PAGE | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus | |
FMN | - |
Rattus norvegicus |