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Literature summary for 1.6.2.4 extracted from

  • Hara, T.; Taniguchi, M.
    Relationship between changes in properties and contents of riboflavin derivatives of NADPH-cytochrome P-450 reductase in the liver microsomes of riboflavin-deficient rats (1985), J. Biochem., 97, 473-482.
    View publication on PubMed
Search for more literature for 1.6.2.4

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Wistar strain
-
Rattus norvegicus Wistar
-
 Wistar strain
-

Purification (Commentary)

Purification (Comment) Organism
column chromatography on DEAE-cellulose and 2',5'-ADP-Sepharose 4B Rattus norvegicus

Renatured (Commentary)

Renatured (Comment) Organism
riboflavin-deficient microsomes, reconstitution of activity with FAD, FMN and riboflavin Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver riboflavin-deficient animals Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.021
-
 riboflavin-deficient animals, feeding period 7 weeks, electron acceptor: cytochrome c Rattus norvegicus
0.066
-
 riboflavin-deficient animals, feeding period 6 weeks, electron acceptor: cytochrome c Rattus norvegicus
0.075
-
 riboflavin deficient animals, feeding period 7 weeks, electron acceptor: ferricyanide Rattus norvegicus
0.097
-
 riboflavin-deficient-animals, feeding period 6 weeks, electron acceptor: ferricyanide Rattus norvegicus
0.157
-
 control animals, feeding period 6 weeks, electron acceptor: ferricyanide Rattus norvegicus
0.165
-
 control animals, feeding period 6 weeks, electron acceptor: cytochrome c Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus Wistar 2 ferrocytochrome c + NADP+ + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rattus norvegicus
FMN riboflavin-deficient microsomes: 40-66% of that of the control microsomes Rattus norvegicus