Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley strain | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley strain | - |
Purification (Comment) | Organism |
---|---|
trypsin digestion followed by Sephadex filtration and column chromatography on DEAE-cellulose | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.025 | - |
electron donor: NADH, electron acceptor: dichlorophenolindophenol | Rattus norvegicus |
0.03 | - |
electron donor: NADPH, electron acceptor: cytochrome b5 | Rattus norvegicus |
0.055 | - |
electron donor: NADH, electron acceptor: cytochrome c | Rattus norvegicus |
17.9 | - |
electron donor: NADPH, electron acceptor: dichlorophenolindophenol | Rattus norvegicus |
37.6 | - |
purified enzyme | Rattus norvegicus |
40.5 | - |
electron donor: NADPH, electron acceptor: ferricyanide | Rattus norvegicus |
41.2 | - |
electron donor: NADPH, electron acceptor: cytochrome c | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-15°C or -20°C, 10 mM phosphate buffer, pH 7.5, several months | Rattus norvegicus |
0°C, some days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Rattus norvegicus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: cytochrome b5 | Rattus norvegicus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Rattus norvegicus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Rattus norvegicus Sprague-Dawley | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: cytochrome b5 | Rattus norvegicus Sprague-Dawley | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Rattus norvegicus Sprague-Dawley | 2 ferrocytochrome c + NADP+ + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | diluted solutions: gradual loss of activity | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus | |
NADH | less effective than NADPH | Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |