Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phenobarbital | stimulation | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Trypanosoma cruzi | |
0.00303 | - |
NADPH | - |
Trypanosoma cruzi | |
0.06289 | - |
ferricytochrome c | - |
Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma cruzi | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Trypanosoma cruzi |
100000 | - |
gel filtration | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose and 2',5'-ADP-agarose column chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
cholate and Lubrol Px | Trypanosoma cruzi |
0.059 | - |
no detergent | Trypanosoma cruzi |
Storage Stability | Organism |
---|---|
-90°C or -20°C, 5-10% loss of activity, reactivation by FAD, 24 h | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Trypanosoma cruzi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: menadione | Trypanosoma cruzi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Trypanosoma cruzi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
ferricyanide + NADPH + H+ | - |
Trypanosoma cruzi | ferrocyanide + NADP+ | - |
r | |
ferricytochrome c + NADPH + H+ | - |
Trypanosoma cruzi | ferrocytochrome c + NADP+ | - |
r | |
NADPH + H+ + menadione | - |
Trypanosoma cruzi | NADP+ + menadiol | - |
r | |
NADPH + H+ oxidized 2,6-dichlorophenolindophenol | - |
Trypanosoma cruzi | ferrocytochrome c + reduced 2,6-dichlorophenolindophenol | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, SDS-PAGE | Trypanosoma cruzi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-90 | - |
inactivation | Trypanosoma cruzi |
2 | 4 | 1 day, inactivation | Trypanosoma cruzi |
20 | - |
1 day, from 5% to 10% loss of activity | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.45 | - |
menadione | - |
Trypanosoma cruzi | |
7.63 | - |
oxidized 2,6-dichlorophenolindophenol | - |
Trypanosoma cruzi | |
15 | - |
ferricytochrome c | - |
Trypanosoma cruzi | |
64.5 | - |
ferricyanide | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol per subunit | Trypanosoma cruzi | |
NADPH | - |
Trypanosoma cruzi |