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Literature summary for 1.6.2.2 extracted from

  • Davis, C.A.; Dhawan, I.K.; Johnson, M.K.; Barber, M.J.
    Heterologous expression of an endogenous rat cytochrome b5/cytochrome b5 reductase fusion protein: Identification of histidines 62 and 85 as the heme axial ligands (2002), Arch. Biochem. Biophys., 400, 63-75.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of a soluble fusion protein comprising a b-type cytochrome containing domain and a FAD-containing domain Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
NADPH cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein Rattus norvegicus
0.007
-
ferricytochrome c cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein Rattus norvegicus
0.008
-
ferricyanide cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADPH
-
Rattus norvegicus 2 ferrocyanide + NADP+ + H+
-
?
2 ferricytochrome c + NADH cytochrome b5/cytochrome b5 reductase FAD-domain-fusion protein, NADPH is preferred Rattus norvegicus 2 ferrocytochrome c + NAD+ + H+
-
?
NADH + ferricytochrome b5
-
Rattus norvegicus NAD+ + H+ + ferrocytochrome b5
-
r
NADH + ferricytochrome c
-
Rattus norvegicus NAD+ + H+ + ferrocytochrome c
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.3
-
ferricytochrome b5 cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein Rattus norvegicus
21.7
-
ferricyanide cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein Rattus norvegicus