Literature summary for 1.6.2.2 extracted from

Kimura, S.; Emi, Y.; Ikushiro, S.; Iyanagi, T.
Systematic mutations of highly conserved His49 and carboxyl-terminal of recombinant porcine liver NADH-cytochrome b5 reductase solubilized domain (1999), Biochim. Biophys. Acta, 1430, 290-301.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzymes in Escherichia coli	Sus scrofa

Protein Variants	Comment	Organism
H49A	reduced kcat	Sus scrofa
H49E	elevated Km value for cytochrome b5, strongly reduced kcat	Sus scrofa
H49K	reduced Km value for cytochrome b5	Sus scrofa
H49Y	similar to wild-type	Sus scrofa

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0003	-	NADH	H49E mutant enzyme	Sus scrofa
0.0006	-	ferricyanide	H49K mutant enzyme	Sus scrofa
0.00064	-	NADH	G273 mutant enzyme	Sus scrofa
0.00084	-	NADH	DELTAF272 mutant enzyme	Sus scrofa
0.0015	-	NADH	recombinant wild-type enzyme	Sus scrofa
0.0016	-	NADH	native enzyme	Sus scrofa
0.0019	-	NADH	H49A mutant enzyme	Sus scrofa
0.0022	-	ferricyanide	H49E mutant enzyme	Sus scrofa
0.0025	-	ferricyanide	native enzyme	Sus scrofa
0.0025	-	cytochrome b5	G273 mutant enzyme	Sus scrofa
0.0026	-	ferricyanide	H49Y mutant enzyme	Sus scrofa
0.0027	-	NADH	H49Y mutant enzyme	Sus scrofa
0.0028	-	ferricyanide	recombinant wild-type enzyme	Sus scrofa
0.0028	-	ferricyanide	H49A mutant enzyme	Sus scrofa
0.0031	-	cytochrome b5	H49K mutant enzyme	Sus scrofa
0.0052	-	ferricyanide	DELTAF272 mutant enzyme	Sus scrofa
0.0052	-	ferricyanide	G273 mutant enzyme	Sus scrofa
0.0088	-	cytochrome b5	native enzyme	Sus scrofa
0.0089	-	cytochrome b5	H49A mutant enzyme	Sus scrofa
0.0091	-	cytochrome b5	recombinant wild-type enzyme	Sus scrofa
0.01	-	NADH	H49K mutant enzyme	Sus scrofa
0.011	-	cytochrome b5	H49Y mutant enzyme	Sus scrofa
0.017	-	cytochrome b5	DELTAF272 mutant enzyme	Sus scrofa
0.03	-	cytochrome b5	H49E mutant enzyme	Sus scrofa

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Comment)	Organism
recombinant wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzyme	Sus scrofa

Source Tissue	Comment	Organism	Textmining

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + ferricytochrome b5	-	Sus scrofa	NAD+ + H+ + ferrocytochrome b5	-	r

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
53	-	NADH	G273 mutant enzyme	Sus scrofa
57	-	ferricytochrome b5	G273 mutant enzyme	Sus scrofa
76	-	ferricyanide	G273 mutant enzyme	Sus scrofa
77	-	NADH	H49E mutant enzyme	Sus scrofa
110	-	ferricyanide	H49E mutant enzyme	Sus scrofa
160	-	ferricytochrome b5	H49E mutant enzyme	Sus scrofa
310	-	NADH	DELTAF272 mutant enzyme	Sus scrofa
380	-	ferricytochrome b5	H49A mutant enzyme	Sus scrofa
390	-	NADH	native enzyme	Sus scrofa
400	-	ferricyanide	DELTAF272 mutant enzyme	Sus scrofa
420	-	ferricytochrome b5	H49K mutant enzyme	Sus scrofa
440	-	NADH	H49A mutant enzyme	Sus scrofa
450	-	ferricytochrome b5	DELTAF272 mutant enzyme	Sus scrofa
470	-	ferricyanide	H49A mutant enzyme	Sus scrofa
490	-	ferricyanide	H49K mutant enzyme	Sus scrofa
510	-	ferricytochrome b5	native enzyme	Sus scrofa
560	-	ferricytochrome b5	recombinant wild-type enzyme	Sus scrofa
570	-	NADH	recombinant wild-type enzyme	Sus scrofa
580	-	ferricytochrome b5	H49Y mutant enzyme	Sus scrofa
630	-	ferricyanide	native enzyme	Sus scrofa
710	-	NADH	H49K mutant enzyme	Sus scrofa
727	-	ferricyanide	H49Y mutant enzyme	Sus scrofa
750	-	NADH	H49Y mutant enzyme	Sus scrofa
810	-	ferricyanide	recombinant wild-type enzyme	Sus scrofa