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Literature summary for 1.6.2.2 extracted from
- Methemoglobin reduction system of erythrocytes (1978), Methods Enzymol., 52, 463-473.
General Stability
| General Stability | Organism |
|---|---|
| enzyme from erythrocyte loses its activity in the absence of EDTA after 5 d at 4°C | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| K+ high ionic strength | reduction of cytochrome b5 or dichlorphenolindophenol | Homo sapiens | |
| Tris | reduction of cytochrome b5 | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00016 | - |
NADH | enzyme from erythrocyte | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | soluble form | Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.5-8.1, 0.5 mM EDTA, protein concentration above 0.02 mg/ml | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Homo sapiens | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 2 ferricytochrome b5 + NADH | poor electron acceptors: methylene blue, ferricytochrome c, O2, oxidized glutathione, methemoglobin | Homo sapiens | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| NADH + ferricytochrome b5 | - |
Homo sapiens | NAD+ + H+ + ferrocytochrome b5 | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.3 | - |
ferricytochrome b5 | - |
Homo sapiens | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.1 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| NADH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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