Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.6.1.4 extracted from

  • Demmer, J.K.; Huang, H.; Wang, S.; Demmer, U.; Thauer, R.K.; Ermler, U.
    Insights into flavin-based electron bifurcation via the NADH-dependent reduced ferredoxin NADP oxidoreductase structure (2015), J. Biol. Chem., 290, 21985-21995 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.225 M NH4H2PO4, 5% (v/v) ethanol, 20% (v/v) glycerol Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains an iron-sulfur center, a [4Fe-4S]-center, and a [2Fe-2S]-center Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + NADP+ + reduced ferredoxin [iron-sulfur] cluster Thermotoga maritima
-
 NAD+ + NADPH + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
NADH + H+ + NADP+ + reduced ferredoxin [iron-sulfur] cluster Thermotoga maritima ATCC 43589
-
 NAD+ + NADPH + oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1X4
-
-
Thermotoga maritima ATCC 43589 Q9X1X4
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin column chromatography and Sephacryl S-200 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + NADP+ + reduced ferredoxin [iron-sulfur] cluster
-
 Thermotoga maritima NAD+ + NADPH + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
NADH + H+ + NADP+ + reduced ferredoxin [iron-sulfur] cluster
-
 Thermotoga maritima ATCC 43589 NAD+ + NADPH + oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 50000 + 1 *30000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
NADH-dependent reduced ferredoxin:NADP oxidoreductase
-
 Thermotoga maritima
NfnAB
-
 Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Thermotoga maritima