Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A432C | the mutant shows altered NADP(H) binding and domain interaction properties | Escherichia coli |
G430C | the mutant shows altered NADP(H) binding and domain interaction properties | Escherichia coli |
R425C | the mutant shows almost exclusively changes in the NADP(H)-binding properties, without changing the affinity for transhydrogenase domain I from Rhodospirillum rubrum | Escherichia coli |
T393C | the mutant shows altered NADP(H) binding and domain interaction properties | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ | Escherichia coli | - |
NADP+ + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ | - |
Escherichia coli | NADP+ + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
proton-translocating transhydrogenase domain III | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |