BRENDA - Enzyme Database
show all sequences of 1.6.1.3

Interactions of the NADP(H)-binding domain III of proton-translocating transhydrogenase from Escherichia coli with NADP(H) and the NAD(H)-binding domain I studied by NMR and site-directed mutagenesis

Bergkvist, A.; Johansson, C.; Johansson, T.; Rydström, J.; Karlsson, g.; Biochemistry 39, 12595-12605 (2000)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Engineering
Protein Variants
Commentary
Organism
A432C
the mutant shows altered NADP(H) binding and domain interaction properties
Escherichia coli
G430C
the mutant shows altered NADP(H) binding and domain interaction properties
Escherichia coli
R425C
the mutant shows almost exclusively changes in the NADP(H)-binding properties, without changing the affinity for transhydrogenase domain I from Rhodospirillum rubrum
Escherichia coli
T393C
the mutant shows altered NADP(H) binding and domain interaction properties
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
NADPH + NAD+
Escherichia coli
-
NADP+ + NADH
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
NADPH + NAD+
-
392667
Escherichia coli
NADP+ + NADH
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
proton-translocating transhydrogenase domain III
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Escherichia coli
Engineering (protein specific)
Protein Variants
Commentary
Organism
A432C
the mutant shows altered NADP(H) binding and domain interaction properties
Escherichia coli
G430C
the mutant shows altered NADP(H) binding and domain interaction properties
Escherichia coli
R425C
the mutant shows almost exclusively changes in the NADP(H)-binding properties, without changing the affinity for transhydrogenase domain I from Rhodospirillum rubrum
Escherichia coli
T393C
the mutant shows altered NADP(H) binding and domain interaction properties
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
NADPH + NAD+
Escherichia coli
-
NADP+ + NADH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
NADPH + NAD+
-
392667
Escherichia coli
NADP+ + NADH
-
-
-
r
Other publictions for EC 1.6.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Nikel
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-
2
-
47
-
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2
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2
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2
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741856
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1
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1
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1
1
-
-
-
392667
Bergkvist
Interactions of the NADP(H)-bi ...
Escherichia coli
Biochemistry
39
12595-12605
2000
-
-
1
-
4
-
-
-
-
-
-
1
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1
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1
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1
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1
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1
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1
1
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4
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1
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1
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1
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-
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724928
Mercer
Hymenolepis diminuta: catalysi ...
Hymenolepis diminuta
Exp. Parasitol.
91
52-58
1999
3
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
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-
-
-
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1
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1
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-
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-
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1
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3
-
-
1
-
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-
-
1
-
-
1
-
-
1
-
-
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1
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1
1
-
-
-
723924
Chen
Malate metabolism by Desulfovi ...
Desulfovibrio gigas
Anaerobe
1
227-235
1995
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-
-
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1
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1
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1
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1
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1
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1
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724104
Vercesi
The participation of NADP, the ...
Rattus norvegicus
Arch. Biochem. Biophys.
252
171-178
1987
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1
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1
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1
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1
1
-
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-
724795
McKelvey
Coupling of malic enzyme and N ...
Hymenolepis diminuta
Comp. Biochem. Physiol. B
77
737-742
1984
-
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-
-
-
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-
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1
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1
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3
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1
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1
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1
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1
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1
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1
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1
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392576
Voordouw
Pyridine nucleotide transhydro ...
Azotobacter vinelandii, Azotobacter vinelandii ATCC 478
Eur. J. Biochem.
111
347-355
1980
-
-
-
-
-
-
-
-
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1
2
-
2
-
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1
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2
-
2
-
1
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2
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2
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1
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1
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2
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2
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722576
Keister
Pyridine nucleotide transhydro ...
Spinacia oleracea
J. Biol. Chem.
235
2989-2996
1960
-
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-
-
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6
-
-
1
-
1
-
1
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1
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-
1
-
1
6
-
-
1
-
2
-
1
1
1
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6
-
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1
-
1
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1
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1
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1
6
-
1
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2
-
1
1
1
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