BRENDA - Enzyme Database
show all sequences of 1.6.1.3

Pyridine nucleotide transhydrogenase from spinach. I. Purification and properties

Keister, D.L.; San Pietro, A.; Stolzenbach, F.E.; J. Biol. Chem. 235, 2989-2996 (1960)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2'-adenylic acid
43% inhibition, activity with NADPH and NAD+
Spinacia oleracea
3'-adenylic acid
37% inhibition, activity with NADPH and NAD+
Spinacia oleracea
5'-adenylic acid
27% inhibition, activity with NADPH and NAD+
Spinacia oleracea
ADP
48% inhibition, activity with NADPH and NAD+
Spinacia oleracea
Alpha-NAD+
33% inhibition, activity with NADPH and NAD+
Spinacia oleracea
ATP
22% inhibition, activity with NADPH and NAD+
Spinacia oleracea
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
1 mM, slight stimulation
Spinacia oleracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
NADPH + NAD+
Spinacia oleracea
the enzyme catalyses the NADPH-driven reduction of NAD+
NADP+ + NADH
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Spinacia oleracea
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Spinacia oleracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Storage Stability
Storage Stability
Organism
-15°C, partially purified, stable for at least one month
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetylpyridine-NADPH + NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
acetylpyridine-NADP+ + NADH
-
-
-
?
deamino-NADPH + NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
deamino-NADP+ + NADH
-
-
-
?
NADPH + acetylpyridine-NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
NADP+ + acetylpyridine-NADH
-
-
-
?
NADPH + deamino-NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
NADP+ + deamino-NADH
-
-
-
?
NADPH + NAD+
the enzyme catalyses the NADPH-driven reduction of NAD+
722576
Spinacia oleracea
NADP+ + NADH
-
-
-
?
NADPH + NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
NADP+ + NADH
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Spinacia oleracea
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
22
-
there is no loss in activity when the enzyme is left overnight at room temperature
Spinacia oleracea
55
-
2.5 min, at least 90% of the activity is retained
Spinacia oleracea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
-
Spinacia oleracea
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.6
9.4
pH 7.6: about 40% of maximal activity, pH 9.4: about 70% of maximal activity
Spinacia oleracea
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
no loss in activity after 15 min, 45% loss of activity after 4 hours
Spinacia oleracea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2'-adenylic acid
43% inhibition, activity with NADPH and NAD+
Spinacia oleracea
3'-adenylic acid
37% inhibition, activity with NADPH and NAD+
Spinacia oleracea
5'-adenylic acid
27% inhibition, activity with NADPH and NAD+
Spinacia oleracea
ADP
48% inhibition, activity with NADPH and NAD+
Spinacia oleracea
Alpha-NAD+
33% inhibition, activity with NADPH and NAD+
Spinacia oleracea
ATP
22% inhibition, activity with NADPH and NAD+
Spinacia oleracea
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
1 mM, slight stimulation
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
NADPH + NAD+
Spinacia oleracea
the enzyme catalyses the NADPH-driven reduction of NAD+
NADP+ + NADH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Spinacia oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, partially purified, stable for at least one month
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetylpyridine-NADPH + NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
acetylpyridine-NADP+ + NADH
-
-
-
?
deamino-NADPH + NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
deamino-NADP+ + NADH
-
-
-
?
NADPH + acetylpyridine-NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
NADP+ + acetylpyridine-NADH
-
-
-
?
NADPH + deamino-NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
NADP+ + deamino-NADH
-
-
-
?
NADPH + NAD+
the enzyme catalyses the NADPH-driven reduction of NAD+
722576
Spinacia oleracea
NADP+ + NADH
-
-
-
?
NADPH + NAD+
stereospecificity with respect to NADPH is not known
722576
Spinacia oleracea
NADP+ + NADH
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Spinacia oleracea
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
22
-
there is no loss in activity when the enzyme is left overnight at room temperature
Spinacia oleracea
55
-
2.5 min, at least 90% of the activity is retained
Spinacia oleracea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
-
Spinacia oleracea
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.6
9.4
pH 7.6: about 40% of maximal activity, pH 9.4: about 70% of maximal activity
Spinacia oleracea
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
no loss in activity after 15 min, 45% loss of activity after 4 hours
Spinacia oleracea
Other publictions for EC 1.6.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742386
Nikel
Pyridine nucleotide transhydr ...
Pseudomonas putida, Pseudomonas putida KT 2240
Environ. Microbiol.
18
3565-3582
2016
-
-
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-
-
-
-
-
1
-
-
2
-
47
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2
-
2
-
-
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-
-
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-
1
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-
2
-
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2
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-
-
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-
-
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-
-
-
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-
741856
Fisher-Wellman
Pyruvate dehydrogenase comple ...
Mus musculus
Biochem. J.
467
271-280
2015
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
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-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
1
-
-
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-
1
-
-
-
-
-
-
-
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-
1
1
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-
-
392667
Bergkvist
Interactions of the NADP(H)-bi ...
Escherichia coli
Biochemistry
39
12595-12605
2000
-
-
1
-
4
-
-
-
-
-
-
1
-
1
-
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1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
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-
-
-
-
1
1
-
4
-
-
-
-
-
-
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-
1
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-
1
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-
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-
1
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-
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-
-
-
-
-
-
-
-
-
-
-
724928
Mercer
Hymenolepis diminuta: catalysi ...
Hymenolepis diminuta
Exp. Parasitol.
91
52-58
1999
3
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
3
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
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-
-
1
-
-
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-
-
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-
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-
1
1
-
-
-
723924
Chen
Malate metabolism by Desulfovi ...
Desulfovibrio gigas
Anaerobe
1
227-235
1995
-
-
-
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-
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1
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1
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1
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1
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1
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1
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1
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-
724104
Vercesi
The participation of NADP, the ...
Rattus norvegicus
Arch. Biochem. Biophys.
252
171-178
1987
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-
-
-
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
1
1
-
-
-
724795
McKelvey
Coupling of malic enzyme and N ...
Hymenolepis diminuta
Comp. Biochem. Physiol. B
77
737-742
1984
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392576
Voordouw
Pyridine nucleotide transhydro ...
Azotobacter vinelandii, Azotobacter vinelandii ATCC 478
Eur. J. Biochem.
111
347-355
1980
-
-
-
-
-
-
-
-
-
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1
2
-
2
-
-
1
-
-
-
2
-
2
-
1
-
-
-
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2
-
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2
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-
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1
2
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1
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2
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2
-
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-
-
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-
-
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-
-
722576
Keister
Pyridine nucleotide transhydro ...
Spinacia oleracea
J. Biol. Chem.
235
2989-2996
1960
-
-
-
-
-
-
6
-
-
1
-
1
-
1
-
-
1
-
-
1
-
1
6
-
-
1
-
2
-
1
1
1
-
-
-
-
-
-
-
-
-
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-
-
6
-
-
-
1
-
1
-
-
-
1
-
1
-
1
6
-
1
-
2
-
1
1
1
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-