Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression and knockdown of the enzyme in carcinoma cells, phenotypes, overview | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Mus musculus | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ | Mus musculus | - |
NADP+ + NADH | - |
r | |
NADPH + NAD+ | Mus musculus C57/BL6J | - |
NADP+ + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus C57/BL6J | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
melanoma cell | - |
Mus musculus | - |
RCC 786-O cell | - |
Mus musculus | - |
renal carcinoma cell | - |
Mus musculus | - |
SK-MEL-5 cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ | - |
Mus musculus | NADP+ + NADH | - |
r | |
NADPH + NAD+ | - |
Mus musculus C57/BL6J | NADP+ + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide nucleotide transhydrogenase | - |
Mus musculus |
NNT | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of the enzyme inhibits the contribution of glutamine to the tricarbonic acid cycle and activates glucose catabolism in SkMel5 melanoma cells. The increase in glucose oxidation partially occurs through pyruvate carboxylase and renders NNT knockdown cells more sensitive to glucose deprivation. Importantly, knocking down NNT inhibits reductive carboxylation in SkMel5 and 786-O renal carcinoma cells. Overexpression of NNT is sufficient to stimulate glutamine oxidation and reductive carboxylation, whereas it inhibits glucose catabolism in the TCA cycle, with impairment of the NAD(P)H/NAD(P)+ ratios | Mus musculus |
physiological function | nicotinamide nucleotide transhydrogenase is a mitochondrial enzyme that transfers reducing equivalents from NADH to NADPH | Mus musculus |